Sini-Suanzaoren decoction regulates mitochondrial biogenesis mediated by MT-SIRT1 in the treatment of insomnia rats

Table 1 Primer sequence of internal reference gene in quantitative real-time PCR

Gene	Forward primer (5′-3′)	Reverse primer (5′-3′)	Product length (bp)
AANAT	CCACCAGTGCGTTTGAGATT	GACACAGGGTGAGGAAGTGC	99
SIRT1	AGGGAACCTCTGCCTCATCT	TGGCATACTCGCCACCTAAC	100
PGC1	GTGCAGCCAAGACTCTGTATGG	GTCCAGGTCATTCACATCAAGTTC	121
NRF1	TATGGCGGAAGTAATGAAAGACG	CAACGTAAGCTCTGCCTTGTT	101
TFAM	ATCAAGACTGTGCGTGCATC	AAAGCCCGGAAGGTTCTTAG	217
GAPDH	CTGGAGAAACCTGCCAAGTATG	GGTGGAAGAATGGGAGTTGCT	138
Cyb	ATCTCACCCCCTATTCAAAATCATC	GGTATCAGACGTGTAGTGTATTGC	160

Table 2 The binding ability of the specific brain-entry ingredients of Sini-Suanzaoren decoction to SIRT1

Formula	Metabolites	Theoretical m/z	m/z	Retention time (min)	Binding energy (kcal/mol)
C48H72O21	licoricesaponin a3	985.4619	985.4629	6.34	-9.5
C12H16O3	Senkyunolide G	209.1170	209.1171	8.02	-8.1
C33H52O5	Pachymic acid	567.3542	567.3494	12.43	-7.8
C20H20O7	Tangeretin	373.1274	373.1278	9.62	-7.3
C12H16O5	Senkyunolide-R	239.0919	239.0922	5.71	-7.0
C18H35NO	9-octadecenamide	326.2679	326.2690	12.53	-6.2
C10H16N4O3	Ophidine	241.1285	241.1290	0.84	-5.4
C9H18N2O3	L-alanyl-L-leucine	203.1390	203.1390	2.73	-5.0