Polymorphic variants in GABA-A receptor and their association with epilepsy and drug resistance: A North Indian cohort study

Table 1 Primers for single nucleotide polymorphism analysis of rs2279020 A/G and rs211013 A/G polymorphism

SNP	Primers	Sequence	Restriction enzyme
rs2279020 A/G	Forward primer	5’ GCTATGGATTGGTTTATTGCCGTGTG 3’	Ava II
	Reverse primer	5’ ATAATATTGATGTACTACAGGGAC 3’
rs211013 A/G	Forward primer	5’ AGAAATTTACCAACTGGTCTAGCCGG 3’	Nci I
	Reverse primer	5’ AAATCAAATATTGTGTCATGCTTAGT 3’

SNP: Single nucleotide polymorphism.

Table 2 Distribution of rs2279020 polymorphism in patients with drug responsive vs drug resistant epilepsy, n (%)

Genotype	Drug responsive (n = 50)	Drug resistant (n = 45)	χ2	P value
AA	30 (60)	30 (66.7)	1.608	0.488
AG	13 (26)	7 (15.6)
GG	7 (14)	5 (11.1)

P value calculated by using χ² test; P < 0.05 considered statistically significant.

Table 3 Risk of drug resistance associated with rs2279020 genotype, n (%)

Genotype	Drug responsive (n = 50)	Drug resistant (n = 45)	Odds ratio (95%CI)	P value
AA	30 (60)	30 (66.7)	Reference	Reference
AG	13 (26)	7 (15.6)	0.966 (0.346-2.698)	0.948
GG	7 (14)	5 (11.1)	1.808 (0.480-6.817)	0.382
AG	13 (26)	7 (15.6)	Reference	Reference
GG	7 (14)	5 (11.1)	1.667 (0.693-4.004)	0.253

P value was calculated using Binary Logistic regression analysis; P < 0.05 considered statistically significant. CI: Confidence interval.