Published online May 19, 2023. doi: 10.5498/wjp.v13.i5.234
Peer-review started: March 9, 2023
First decision: March 23, 2023
Revised: March 31, 2023
Accepted: April 7, 2023
Article in press: April 7, 2023
Published online: May 19, 2023
Processing time: 71 Days and 2.8 Hours
The Borg’s rating of perceived exertion (BRPE) scale was widely used to access subjective perception of effort during exercise, but the relationship between BRPE scale and urinary organic acids metabolism has not been studied.
Our article mainly explored the relationship of urinary organic acids metabolism and the BRPE scale during exercise and reflected the psychological perception degree of effort from an objective physiological perspective.
In this work, we aimed to evaluate whether the BRPE scale could be used in the prescription of outdoor hiking with weight-bearing based on urinary organic acids metabolism, which provides an objective physiological data support for the application of BRPE scale in outdoor hiking training.
Eighty-nine healthy men participated in this project and underwent 40 km (6 h) training with 20 kg carriages. After the training, they truthfully filled in the BRPE scale and urinary organic acids were detected. We used multidimensional statistical analysis including principal component (PCA) analysis and orthogonal partial least-squares discrimination (OPLS-DA) analysis and heat map analysis to explore the differences in metabolic profiles of organic acids with different BRPE scale. At last, differential metabolites were screened and pathway analysis was performed.
There were significant statistical differences in urinary organic acids before and after exercise. According to the BRPE scale, individuals were divided into groups of 6-12 scale (easy), 13-16 scale (somewhat hard) and 17-20 scale (very hard). PCA results showed no separation trend among the three groups. Further analysis with OPLS-DA showed that, group of 6-12 scale and 13-16 scale, 6-12 scale and 17-20 scale could be separated obviously, and group of 13-16 scale and 17-20 scale could not be separated, so group of 13-16 scale and 17-20 scale were combined into group 13-20 scale. OPLS-DA results showed that group 6-12 scale and 13-20 scale could be separated. Heat map results also showed significant metabolic differences between group of 6-12 scale and 13-20 scale. According to the standard of a variable importance in the projection > 1, fold change > 1.5 and P < 0.05, 19 different metabolites were screened, which mainly in citrate cycle (tricarboxylic acid cycle) and alanine, aspartate and glucose metabolism.
Our results showed that the BRPE scale could be used to monitor body fatigue in long-distance outdoor hiking with weight bearing.
We provide an objective method to evaluate body fatigue in outdoor-hiking exercise.