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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.
World J Clin Pediatr. Jun 9, 2026; 15(2): 112072
Published online Jun 9, 2026. doi: 10.5409/wjcp.v15.i2.112072
Impact of breast milk composition on nutritional status of term neonates with congenital heart defects: A prospective study
Aleksandra V Kaplina, Tatiana A Fedoseeva, Tatiana M Pervunina, Olga V Kalinina, Olga O Shemyakina, Tatiana G Barakova, Maria V Kim, Natalia A Petrova
Aleksandra V Kaplina, Tatiana A Fedoseeva, Tatiana M Pervunina, Natalia A Petrova, Institution of Perinatology and Pediatrics, Almazov National Medical Research Centre, Saint-Petersburg 197341, Russia
Olga V Kalinina, Institute of Molecular Biology and Genetics, Department of Laboratory Medicine with Clinic, Almazov National Medical Research Centre, Saint-Petersburg 197341, Russia
Olga O Shemyakina, Tatiana G Barakova, Maria V Kim, Department of Neonatal Physiology with an ICU Ward, Almazov National Medical Research Centre, Saint-Petersburg 197341, Russia
Author contributions: Kaplina AV designed and conducted the study, collected the data, analyzed and interpreted the data, and drafted the initial manuscript; Fedoseeva TA contributed to the analyses; Barakova TG and Kim MV provided clinical advice; Pervunina TM and Petrova NA contributed to project administration and funding acquisition; Kalinina OV participated in data analysis and interpretation; Shemyakina OO designed the study; Petrova NA designed and supervised the study and drafted the initial manuscript; All authors contributed to manuscript editing and approved the final version of the manuscript.
Supported by Ministry of Health of Russia, No. LPUH-2025-0054.
Institutional review board statement: This study was approved by the local institutional Ethics Committee (protocol No. 1505-25) and conducted in accordance with the principles outlined in the Declaration of Helsinki.
Informed consent statement: Written informed consent was obtained from all mothers who participated in the study.
Conflict-of-interest statement: The authors declare no competing interests.
CONSORT 2010 statement: The authors have read the CONSORT 2010 Statement, and the manuscript was prepared and revised according to the CONSORT 2010 Statement.
Data sharing statement: No additional data are available.
Corresponding author: Aleksandra V Kaplina, Institution of Perinatology and Pediatrics, Almazov National Medical Research Centre, Akkuratova Str 2, Saint-Petersburg 197341, Russia. kaplinashi@gmail.com
Received: July 17, 2025
Revised: August 25, 2025
Accepted: December 1, 2025
Published online: June 9, 2026
Processing time: 300 Days and 22 Hours
Abstract
BACKGROUND

Congenital heart defects (CHDs) are the most common congenital malformations, often requiring surgical intervention during the early neonatal period. Although most children with CHDs are born at full term with appropriate birth weights, many of them develop malnutrition during the first weeks of life, resulting in complications after cardiac surgery. This study aimed to provide new insights into personalized nutritional interventions based on variations in breast milk (BM) composition. We hypothesized that the nutritional and energy composition of BM is associated with the growth trajectory during the late neonatal period in neonates with CHDs, and it may guide individualized nutrition support.

AIM

To assess the BM composition in mothers of neonates with CHD and evaluate its role in the nutritional status of these neonates.

METHODS

In this single-center prospective non-interventional study, we analyzed the BM composition in 35 mothers of neonates with CHDs (25 operated, 10 non-operated) at V1 (2-5 days), V2 (7-12), and V3 (14-40), and also in 21 mothers of healthy infants at V1 (n = 21) and V2 (n = 12), using the Miris Human Milk Analyzer. The weight-for-age z score (WAZ) at discharge was assessed in infants with CHD. Associations between the BM composition, nutrient intake, postoperative course, and WAZ change were evaluated.

RESULTS

Only the colostrum fat concentration was significantly lower in mothers of operated neonates [2.4 (2.0-2.6) g/100 mL] compared with mothers of non-operated neonates [2.9 (2.2-3.1) g/100 mL] and controls [2.7 (2.3-3.3) g/100 mL] (P = 0.045). Operated neonates exhibited a greater decrease in WAZ from birth to discharge than non-operated neonates (P = 0.008). Postoperative WAZ decline was associated with the postoperative period severity and delayed reintroduction of BM feeding. Higher calorie and fat intake on postoperative day 14 was significantly associated with a smaller WAZ decrease (P < 0.05). Later postoperative achievement of full enteral feeding independently predicted WAZ decline. In the operated group the colostrum protein content positively correlated with weight gain during the early neonatal period.

CONCLUSION

Colostrum from mothers of operated neonates with CHD had reduced fat content. BM composition did not affect WAZ trajectories. However, delayed achievement of full enteral feeding independently predicted WAZ decline.

Keywords: Breast milk composition; Congenital heart defects; Term neonates; Nutrition; Weight-for-age z score trajectories; Enteral feeding

Core Tip: This single-center prospective non-interventional study evaluated breast milk composition in mothers of neonates with congenital heart defects and its relationship with neonatal nutritional status. The colostrum fat concentration was significantly lower in mothers of operated neonates than in mothers of non-operated neonates. Operated neonates showed a decline in weight-for-age z score (WAZ) from preoperative values to discharge. The delayed achievement of full enteral feeding postoperatively independently predicted WAZ decline while higher calorie and fat intake on postoperative day 14 was associated with less WAZ reduction. In operated neonates higher colostrum protein content correlated with early weight gain.

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