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©The Author(s) 2025.
World J Clin Oncol. Sep 24, 2025; 16(9): 111742
Published online Sep 24, 2025. doi: 10.5306/wjco.v16.i9.111742
Published online Sep 24, 2025. doi: 10.5306/wjco.v16.i9.111742
Table 1 Primers for target genes of miR-451a
| Gene ID | Forward primer sequence | Reverse primer sequence | Amplification fragment (bp) |
| GAPDH | TGACTTCAACAGCGACACCCA | CACCCTGTTGCTGTAGCCAAA | 121 |
| TSC1 | CAACAAGCAAATGTCGGGGAG | CATAGGGCCACGGTCAGAA | 114 |
| OSR1 | CACAGTGCCTTCGGACCATCT | CTCGGGCTTGGGTTGAATGAC | 211 |
| EMSY | ATGACTTCCAAGCCCAACGTG | TTCCCTTGCTGCCCATAAACA | 275 |
| SAMD4B | TGGGCATTCGGCTCCAACTCG | CATTGGGAACTGCCGCTGGGT | 84 |
| MEX3C | ATGAGGTTATTGCTGCCCTAG | GTGAATTTGGATTGCCTGAGTA | 134 |
| PSMB8 | CTGGGTCCTACATTAGTGCCT | CCATTTCGCAGATAGTACAGC | 148 |
| PMM2 | ATCAAAATCGGAGTGGTAGGC | CAGCTTCTTCCAATAGGGGAC | 293 |
| CAB39 | GACAAAAGTCGCAACATCCAG | TAAACTGCTCATCCTCCGTCC | 166 |
| TTN | GGGAGGAAAGGCTTACAGAG | ATGGATACCATCATAGCGAACT | 261 |
Table 2 Expression of miR-451a in hepatocellular carcinoma cell lines
| Cell type | U6 | hsa-miR-451a, Ct value | ∆Ct |
| Human hepatoblastoma G2 | 10.84 | 35 | 24.16 |
| 10.72 | 35 | 24.28 | |
| 10.77 | 35 | 24.23 | |
| BEL-7402 | 13.19 | 35 | 21.81 |
| 13.09 | 35 | 21.91 | |
| 13.2 | 35 | 21.8 | |
| BEL-7404 | 11.67 | 35 | 23.33 |
| 11.61 | 35 | 23.39 | |
| 11.53 | 35 | 23.47 | |
| Huh-7 | 10.82 | 35 | 24.18 |
| 10.84 | 35 | 24.16 | |
| 10.74 | 35 | 24.26 | |
| SMMC-7721 | 12.56 | 35 | 22.44 |
| 12.58 | 35 | 22.42 | |
| 12.66 | 35 | 22.34 | |
| Hep3B | 13.55 | 35 | 21.45 |
| 13.53 | 35 | 21.47 | |
| 13.51 | 35 | 21.49 |
Table 3 Expression of miR-451a in colorectal cancer cell and esophageal squamous cancer cell
| Cell type | U6 | hsa-miR-451a, Ct value | ∆Ct |
| HCT116 | 11.74 | 35 | 23.26 |
| 11.56 | 35 | 23.44 | |
| 11.68 | 35 | 23.32 | |
| RKO | 11.77 | 35 | 23.23 |
| 11.7 | 35 | 23.3 | |
| 11.79 | 35 | 23.21 | |
| ECA109 | 11.04 | 35 | 23.96 |
| 11.07 | 35 | 23.93 | |
| 11.06 | 35 | 23.94 | |
| TE-1 | 11.79 | 35 | 23.21 |
| 11.81 | 35 | 23.19 | |
| 11.79 | 35 | 23.21 |
- Citation: Liu DZ, Wang L, Yang SK, Zhao ZJ, Cui YY, Zhao XK, Zhang F, Guan QH, Zhou L. MiR-451a targets mex-3 RNA binding family member C to regulate human hepatoblastoma G2 cell growth and metastasis. World J Clin Oncol 2025; 16(9): 111742
- URL: https://www.wjgnet.com/2218-4333/full/v16/i9/111742.htm
- DOI: https://dx.doi.org/10.5306/wjco.v16.i9.111742
