MiR-451a targets mex-3 RNA binding family member C to regulate human hepatoblastoma G2 cell growth and metastasis

Table 1 Primers for target genes of miR-451a

Gene ID	Forward primer sequence	Reverse primer sequence	Amplification fragment (bp)
GAPDH	TGACTTCAACAGCGACACCCA	CACCCTGTTGCTGTAGCCAAA	121
TSC1	CAACAAGCAAATGTCGGGGAG	CATAGGGCCACGGTCAGAA	114
OSR1	CACAGTGCCTTCGGACCATCT	CTCGGGCTTGGGTTGAATGAC	211
EMSY	ATGACTTCCAAGCCCAACGTG	TTCCCTTGCTGCCCATAAACA	275
SAMD4B	TGGGCATTCGGCTCCAACTCG	CATTGGGAACTGCCGCTGGGT	84
MEX3C	ATGAGGTTATTGCTGCCCTAG	GTGAATTTGGATTGCCTGAGTA	134
PSMB8	CTGGGTCCTACATTAGTGCCT	CCATTTCGCAGATAGTACAGC	148
PMM2	ATCAAAATCGGAGTGGTAGGC	CAGCTTCTTCCAATAGGGGAC	293
CAB39	GACAAAAGTCGCAACATCCAG	TAAACTGCTCATCCTCCGTCC	166
TTN	GGGAGGAAAGGCTTACAGAG	ATGGATACCATCATAGCGAACT	261

Table 2 Expression of miR-451a in hepatocellular carcinoma cell lines

Cell type	U6	hsa-miR-451a, Ct value	∆Ct
Human hepatoblastoma G2	10.84	35	24.16
	10.72	35	24.28
	10.77	35	24.23
BEL-7402	13.19	35	21.81
	13.09	35	21.91
	13.2	35	21.8
BEL-7404	11.67	35	23.33
	11.61	35	23.39
	11.53	35	23.47
Huh-7	10.82	35	24.18
	10.84	35	24.16
	10.74	35	24.26
SMMC-7721	12.56	35	22.44
	12.58	35	22.42
	12.66	35	22.34
Hep3B	13.55	35	21.45
	13.53	35	21.47
	13.51	35	21.49

Table 3 Expression of miR-451a in colorectal cancer cell and esophageal squamous cancer cell

Cell type	U6	hsa-miR-451a, Ct value	∆Ct
HCT116	11.74	35	23.26
	11.56	35	23.44
	11.68	35	23.32
RKO	11.77	35	23.23
	11.7	35	23.3
	11.79	35	23.21
ECA109	11.04	35	23.96
	11.07	35	23.93
	11.06	35	23.94
TE-1	11.79	35	23.21
	11.81	35	23.19
	11.79	35	23.21