Identification of SRSF2 as a potential immunodiagnostic biomarker for osteosarcoma: A study based on focused protein microarray

Figure 1 Receiver operator characteristic curve and scatter plots for three anti-tumor associated antigen autoantibodies in the cohort for focused protein microarray. A-C: Analysis of receiver operator characteristic curve with area under the curve and 95% confidence interval; D-F: Serological levels in signal to noise ratio of three anti-tumor associated antigen autoantibodies in osteosarcoma and normal human sera in scatter plots. The longest line means median, and the 25^th percentiles and 75^th percentiles are presented by the shorter line. GNA11: G-protein subunit alpha 11; AUC: Area under the curve; CI: Confidence interval; SRSF2: Serine/arginine-rich splicing factor 2; PIK3CA: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; SNR: Signal to noise ratio; OS: Osteosarcoma; NHS: Normal human sera.

Figure 2 Receiver operator characteristic curve and scatter plots for three anti-tumor associated antigen autoantibodies in the validation cohort. A-C: Analysis of receiver operator characteristic curve with area under the curve and 95% confidence interval; D-F: Expression levels of optical density about three anti-tumor associated antigen autoantibodies in osteosarcoma, osteochondroma, and normal controls in scatter plots. The longest line means median, and the 25^th percentiles and 75^th percentiles are presented by the shorter line. ^aP < 0.05. GNA11: G-protein subunit alpha 11; AUC: Area under the curve; CI: Confidence interval; SRSF2: Serine/arginine-rich splicing factor 2; PIK3CA: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha; OS: Osteosarcoma; NC: Normal control; OC: Osteochondroma; OD: Optical density.

Figure 3 Serine/arginine-rich splicing factor 2 is overexpressed in osteosarcoma tissue compared with the osteochondroma and normal bone tissue by immunohistochemistry. A and B: Positive staining of serine/arginine-rich splicing factor 2 (SRSF2) expressed in representative osteosarcoma tissue at 100 × and 400 × magnification, respectively; C and D: Weak positive staining of SRSF2 expressed in osteochondroma tissue at 100 × and 400 × magnification, respectively; E and F: Negative staining of SRSF2 expressed in normal bone tissue at 100 × and 400 × magnification, respectively; G: Immunohistochemistry. Scores of SRSF2 between osteosarcoma, osteochondroma, and normal controls. IHC: Immunohistochemistry; SRSF2: Serine/arginine-rich splicing factor 2; OS: Osteosarcoma; OC: Osteochondroma; NC: Normal control.