Helicobacter pylori infection promotes the progression of gastric cancer by regulating the expression of DMBT1

Figure 1 Quantitative real-time polymerase chain reaction to detect the relative expression of deleted in malignant brain tumors 1 mRNA in AGS cells of each group. ^bP < 0.001, ^cP < 0.0001. H. pylori: Helicobacter pylori; DMBT1: Deleted in malignant brain tumors 1.

Figure 2 Western blot detection of the relative expression of deleted in malignant brain tumors 1 mRNA in AGS cells of each group. ^aP < 0.01, ^bP < 0.001. H. pylori: Helicobacter pylori; DMBT1: Deleted in malignant brain tumors 1; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Figure 3 Cell counting kit-8, scratch, and Transwell assays to detect the biological behavior in AGS cells of each group. A: Cell counting kit-8 assay was employed to detect the proliferation ability of AGS cells in each group; B and C: Cell scratch assay to detect the migration ability of AGS cells in each group (40 ×); D and E: Transwell assay was performed in AGS cells to detect the invasion ability in each group (40 ×). ^bP < 0.001,^cP < 0.0001. H. pylori: Helicobacter pylori; DMBT1: Deleted in malignant brain tumors 1; OD: Optical density.