Hub genes and their key effects on prognosis of Burkitt lymphoma

Figure 1 Clustering tree of 36 samples of Burkitt lymphoma extracted from GSE4475. Red indicated more gene expression, white less, and grey indicated deletion. CCS: Chromosomal Complexity Score.

Figure 2 Sample clustering to detect outliers and construction of co-expression modules. A: The constructed co-expression modules of Burkitt lymphoma genes by weighted gene co-expression network analysis; B: Interaction analysis between gene co-expression modules. The heatmap showed the Topological Overlap Matrix among genes in the analysis. Different colors on the x-axis and y-axis represented different modules. The intensity of inter-module connections was visually represented by the yellow brightness in the central region, gradually transitioning into deeper shades of orange.

Figure 3 Module-trait association. Correlation thermography between modular feature genes and clinical features of Burkitt lymphoma. Each row corresponded to a module feature, and the column corresponded to a clinical feature. Each cell contained the correlation and the corresponding P value. CCS: Chromosomal Complexity Score; ME: Module membership.

Figure 4 The scatter plot of the correlation for an age-related gene between module membership and gene significance in the yellow module.

Figure 5 Genetic and Protein-Protein interaction network of hub genes. A: GeneMANIA was used to construct a genetic interaction network. The black nodes with a slash represent the query gene, while the other nodes represent the predicted genes. The purple edges indicate co-expression, whereas the blue edges signify co-localization; B: A physically and functionally connected Protein-Protein Interaction network implemented common goals through Search Tool for the Retrieval of Interacting Genes/Proteins, where nodes represented proteins and edges represented pairs of interactions between proteins. Node size and color indicated richness, while edge size and color reflected combined scores.

Figure 6 Functional enrichment analysis results of hub genes. A: The top 10 gene ontology terms of hub genes; B: The top 10 Kyoto Encyclopedia of Genes and Genomes pathways of hub genes. GO: Gene Ontology; KEGG: Kyoto Encyclopedia of Genes and Genomes.

Figure 7 Kaplan–Meier survival curve. A to J: Kaplan–Meier survival curve of identified hub genes in GSE69051.

Figure 8 Nomogram and calibration plot for GSE69051 cohort. A: The nomogram was constructed to predicting1, 3-year survival rate of Burkitt lymphoma patients; B: The calibration curves for predicting patient survival at 1 and 3 years in the cohort. OS: Overall survival.

Figure 9 Drugs related to IL2RA and CXCL10.