Basic Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Pathophysiol. Jan 22, 2021; 12(1): 1-13
Published online Jan 22, 2021. doi: 10.4291/wjgp.v12.i1.1
Influence of anastomoses on intestine ischemia and cefuroxime concentrations: Evaluated in the ileum and colon in a porcine model
Pelle Hanberg, Mats Bue, Maja Thomassen, Uffe Schou Løve, Josephine Olsen Kipp, Christina Harlev, Elisabeth Petersen, Kjeld Søballe, Maiken Stilling
Pelle Hanberg, Department of Orthopaedic Surgery, Horsens Regional Hospital, Horsens 8700, Denmark
Pelle Hanberg, Mats Bue, Maja Thomassen, Josephine Olsen Kipp, Christina Harlev, Elisabeth Petersen, Kjeld Søballe, Maiken Stilling, Aarhus Microdialysis Research Group, Orthopaedic Research Unit, Aarhus University Hospital, Aarhus N 8200, Denmark
Pelle Hanberg, Mats Bue, Kjeld Søballe, Maiken Stilling, Department of Clinical Medicine, Aarhus University, Aarhus N 8200, Denmark
Mats Bue, Kjeld Søballe, Maiken Stilling, Department of Orthopaedic Surgery, Aarhus University Hospital, Aarhus N 8200, Denmark
Uffe Schou Løve, Department of Surgery, Viborg Regional Hospital, Viborg 8800, Denmark
Author contributions: Hanberg P, Bue M, Thomassen M, Løve US, Søballe K and Stilling M contributed study conception design; Hanberg P, Bue M, Thomassen M, Kipp JO, Harlev C and Petersen E contributed data acquisition; Hanberg P contributed data analysis and interpretation, and drafted the article; Hanberg P, Bue M, Thomassen M, Løve US, Kipp JO, Harlev C, Petersen E, Søballe K and Stilling M contributed critical revision; all authors have read and approved the final manuscript.
Institutional review board statement: The study was reviewed and approved by the Danish National Committee and the Animal Experiments Inspectorate.
Institutional animal care and use committee statement: The study was carried out according to existing laws and approved by the Danish Animal Experiments Inspectorate (license No.: 2017/15-0201-01184). All appropriate measures were taken to minimize animal pain and discomfort.
Conflict-of-interest statement: No conflict of interest.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at pellehanberg@clin.au.dk.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Corresponding author: Pelle Hanberg, MD, Department of Orthopaedic Surgery, Horsens Regional Hospital, Sundvej 30, Horsens 8700, Denmark. pellehanberg@clin.au.dk
Received: October 26, 2020
Peer-review started: October 26, 2020
First decision: November 20, 2020
Revised: November 30, 2020
Accepted: December 8, 2020
Article in press: December 8, 2020
Published online: January 22, 2021
Processing time: 86 Days and 23.9 Hours
Core Tip

Core Tip: We found that only colon anastomosis was locally vulnerable to ischemia but reached similar cefuroxime concentrations to those in the remaining investigated intestine compartments. Our study suggests that administering 1.5 g cefuroxime 10 min prior to intestine surgery is sufficient, and that effective concentrations are sustained for approximately 2 h. This is the first study to investigate the influence of anastomoses on ileum and colon ischemic metabolites and cefuroxime concentrations in a simultaneous paired design.