Kurabe N, Igarashi H, Ohnishi I, Tajima S, Inoue Y, Takahashi Y, Setou M, Sugimura H. Visualization of sphingolipids and phospholipids in the fundic gland mucosa of human stomach using imaging mass spectrometry. World J Gastrointest Pathophysiol 2016; 7(2): 235-241 [PMID: 27190696 DOI: 10.4291/wjgp.v7.i2.235]
Corresponding Author of This Article
Haruhiko Sugimura, MD, PhD, Professor, Department of Tumor Pathology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-Ku, Hamamatsu, Shizuoka 431-3192, Japan. hsugimur@hama-med.ac.jp
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Share the Article
Kurabe N, Igarashi H, Ohnishi I, Tajima S, Inoue Y, Takahashi Y, Setou M, Sugimura H. Visualization of sphingolipids and phospholipids in the fundic gland mucosa of human stomach using imaging mass spectrometry. World J Gastrointest Pathophysiol 2016; 7(2): 235-241 [PMID: 27190696 DOI: 10.4291/wjgp.v7.i2.235]
Nobuya Kurabe, Hisaki Igarashi, Ippei Ohnishi, Yusuke Inoue, Yoshihiko Takahashi, Haruhiko Sugimura, Department of Tumor Pathology, Hamamatsu University School of Medicine, Shizuoka 431-3192, Japan
Ippei Ohnishi, Division of Pathology, Iwata City Hospital, Shizuoka 438-8550, Japan
Shogo Tajima, Department of Pathology, the University of Tokyo, Tokyo 113-0033, Japan
Yusuke Inoue, Second Division, Department of Internal Medicine, Hamamatsu University School of Medicine, Shizuoka 431-3192, Japan
Mitsutoshi Setou, Department of Cell Biology and Anatomy, Hamamatsu University School of Medicine, Shizuoka 431-3192, Japan
Author contributions: Kurabe N, Igarashi H, Ohnishi I, Tajima S, Inoue Y, Setou M and Sugimura H substantially contributed to the conception and design of the study and acquisition, analysis, and interpretation of data; all authors drafted the manuscript, made critical revisions related to the intellectual content of the manuscript, and approved the final version of the manuscript to be publishied.
Institutional review board statement: All the gastric tissue specimens were residual tissues from surgical procedures. The patients gave informed consent for research use, provided that the study was conducted anonymously.
Conflict-of-interest statement: To the best of our knowledge, no conflict of interest exists.
Data sharing statement: No additional data are available.
Correspondence to: Haruhiko Sugimura, MD, PhD, Professor, Department of Tumor Pathology, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-Ku, Hamamatsu, Shizuoka 431-3192, Japan. hsugimur@hama-med.ac.jp
Telephone: +81-53-4352220 Fax: +81-53-4352225
Received: December 10, 2015 Peer-review started: December 11, 2015 First decision: January 13, 2016 Revised: January 21, 2016 Accepted: March 1, 2016 Article in press: March 2, 2016 Published online: May 15, 2016 Processing time: 154 Days and 1.7 Hours
Abstract
AIM: To analyze the lipid distribution in gastric mucosae.
METHODS: Imaging mass spectrometry (MS) is a useful tool to survey the distribution of biomolecules in surgical specimens. Here we used the imaging MS apparatus named iMScope to identify the dominant molecules present in the human gastric mucosa near the fundic glands. Five gastric specimens were subjected to iMScope analysis. These specimens were also analyzed by immunohistochemistry using MUC5AC, H(+)-K(+)-ATPaseβ Claudin18 antibodies.
RESULTS: Three major molecules with m/z 725.5, 780.5, and 782.5 detected in the gastric mucosa were identified as sphingomyelin (SM) (d18:1/16:0), phosphatidylcholine (PC) (16:0/18:2), and PC (16:0/18:1), respectively, through MS/MS analyses. Using immunohistological staining, SM (d18:1/16:0) signals were mainly co-localized with the foveolar epithelium marker MUC5AC. In contrast, PC (16:0/18:2) signals were observed in the region testing positive for the fundic gland marker H(+)-K(+)-ATPaseβ. PC (16:0/18:1) signals were uniformly distributed throughout the mucosa.
CONCLUSION: Our basic data will contribute to the studies of lipid species in physical and pathological conditions of the human stomach.
Core tip: Imaging mass spectrometry (MS) is a useful tool to survey the distribution of biomolecules in surgical specimens. Here we used the imaging MS apparatus named iMScope to identify the dominant molecules present in the human gastric mucosa near the fundic glands. Three major molecules with m/z 725.5, 780.5, and 782.5 detected in the gastric mucosa were identified as sphingomyelin (d18:1/16:0), phosphatidylcholine (PC) (16:0/18:2), and PC (16:0/18:1), respectively.
