Câmara PR, Ferraz GJ, Velloso LA, Zeitune JMR, Suassuna FA, Ferraz JGP. Endothelin and neonatal capsaicin regulate gastric resistance to injury in BDL rats. World J Gastrointest Pathophysiol 2012; 3(4): 85-91 [PMID: 23293745 DOI: 10.4291/wjgp.v3.i4.85]
Corresponding Author of This Article
Paula RS Câmara, PhD, Department of Medicine, Potiguar University, Av. Senador Salgado Filho, 1610, 59056-000 - Natal, RN, Brazil. paula.camara@unp.br
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Original Article
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World J Gastrointest Pathophysiol. Aug 15, 2012; 3(4): 85-91 Published online Aug 15, 2012. doi: 10.4291/wjgp.v3.i4.85
Endothelin and neonatal capsaicin regulate gastric resistance to injury in BDL rats
Paula RS Câmara, Gerson JN Ferraz, Licio A Velloso, José Murilo R Zeitune, Fernando AB Suassuna, Jose Geraldo P Ferraz
Paula RS Câmara, Fernando AB Suassuna, Department of Medicine, Potiguar University, RN 59056-000, Brazil
Paula RS Câmara, Gerson JN Ferraz, Licio A Velloso, José Murilo R Zeitune, Jose Geraldo P Ferraz, GI Research Lab, Gastrocentro, Department of Medicine, Faculty of Medical Sciences, University of Campinas, SP 13083-878, Brazil
Jose Geraldo P Ferraz, Division of Gastroenterology, Faculty of Medicine, University of Calgary, Calgary, AB T2N 4N1, Canada
Author contributions: Câmara PRS, Ferraz JGP, Velloso LA and Zeitune JMR designed the research; Câmara PRS and Ferrazs GJN performed the research; Câmara PRS, Suassuna FAB, Ferrazs GJN and Zeitune JMR analyzed the data; Câmara PRS and Ferrazs GJN wrote the paper; Zeitune JMR reviewed it.
Supported by A fellowship from Fundaçao de Amparo a Pesquisa do Estado de Sao Paulo, FAPESP, Brazil (to Câmara PRS) and a research grant from FAPESP (to Ferraz JGP)
Correspondence to: Paula RS Câmara, PhD, Department of Medicine, Potiguar University, Av. Senador Salgado Filho, 1610, 59056-000 - Natal, RN, Brazil. paula.camara@unp.br
Telephone: +55-84-32151273 Fax: +55-84-32151200
Received: January 14, 2012 Revised: August 6, 2012 Accepted: August 10, 2012 Published online: August 15, 2012
Abstract
AIM: To investigate the relationship between primary afferent neurons, endothelin (ET) and the role of its receptors on ethanol-induced gastric damage in cirrhotic rats.
METHODS: Cirrhosis and portal hypertension were induced in rats by bile duct ligation (BDL) while controls had a sham operation. The association between ET and afferent neurons on the gastric mucosa was evaluated by capsaicin treatment in newborn rats, the use of ET agonists or antagonists, gastric ET-1 and -3 mRNA and synthetic capacity. Ethanol-induced damage was assessed using ex vivo gastric chamber experiments. Gastric blood flow was measured by laser-Doppler flowmetry.
RESULTS: ET-3 and an ETB receptor antagonist significantly reduced the extent of ethanol-induced gastric damage in BDL rats. Gastric ET-1 and -3 levels were 30% higher in BDL rats compared to control rats. Capsaicin treatment restored the gastric resistance and blood flow responses to topical application of ethanol in BDL rats and ET-1 and -3 production to levels observed in controls.
CONCLUSION: Our results suggest that the reduced resistance of the gastric mucosa of cirrhotic rats to ethanol-induced injury is a phenomenon modulated by ET through the ETB receptor and by sensory afferent neurons.
