Basic Study
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Cardiol. Feb 26, 2025; 17(2): 102310
Published online Feb 26, 2025. doi: 10.4330/wjc.v17.i2.102310
Assessment of the biosafety profile of Galium verum in vitro on myoblasts and in ovo on chorioallantoic membrane
Diana Maria Morariu-Briciu, Sorin Lucian Bolintineanu, Andreea Rata, Alexandra Denisa Semenescu, Alina Anton, Robert Jijie, Andreea Kis, Ingrid Hrubaru, Alina Heghes
Diana Maria Morariu-Briciu, Sorin Lucian Bolintineanu, Department of Anatomy and Embryology, “Victor Babes” University of Medicine and Pharmacy, Timisoara 300041, Romania
Diana Maria Morariu-Briciu, Doctoral School, “Victor Babes” University of Medicine and Pharmacy, Timisoara 300041, Romania
Andreea Rata, Department of Vascular Surgery, “Victor Babes” University of Medicine and Pharmacy, Timisoara 300041, Romania
Alexandra Denisa Semenescu, Alina Anton, Robert Jijie, Andreea Kis, Department of Toxicology, Drug Industry, Management and Legislation, “Victor Babes” University of Medicine and Pharmacy, Timisoara 300041, Romania
Alexandra Denisa Semenescu, Alina Anton, Robert Jijie, Andreea Kis, Research Center for Pharmaco-Toxicological Evaluations, “Victor Babes” University of Medicine and Pharmacy, Timisoara 300041, Romania
Ingrid Hrubaru, Department of Obstetrics and Gynecology, “Victor Babes” University of Medicine and Pharmacy, Timisoara 300041, Romania
Alina Heghes, Department of Pharmaceutical Technology, “Victor Babes” University of Medicine and Pharmacy, Timisoara 300041, Romania
Author contributions: Morariu-Briciu DM, Bolintineanu SL, Rata A, Anton A, and Heghes A designed and coordinated the study; Morariu-Briciu DM, Semenescu AD, Jijie R, Kis A, and Hrubaru I performed the experiments and acquired and analyzed data; Morariu-Briciu DM, Anton A, Heghes A, Rata A, Bolintineanu SL, Semenescu AD, Jijie R, Kis A, and Hrubaru I interpreted the data; Morariu-Briciu DM, Bolintineanu SL, Heghes A, Anton A, Hrubaru I, Semenescu A, Rata A, Jijie R, and Kis A wrote the manuscript; All authors approved the final version of the article.
Institutional review board statement: The current study was conducted within the research center for Pharmacotoxicological Evaluations, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu Sq. No. 2, 300041 Timisoara, Romania. In vitro and in ovo studies did not require any special Institutional Review Board Approval.
Institutional animal care and use committee statement: The study presented in manuscript 102310 was performed using two types of experimental models, namely in vitro (e.g., cell line) and in ovo (e.g., fertilized chicken eggs). An ethics committee approval was not necessary for the present in vitro study, as the cell line used for the conducted experiments was commercially obtained from licensed manufacturers and cultured according to their recommendations (H9-C2(2-1), from ATCC CRL-1446™, https://www.atcc.org/products/crl-1446). In addition, regarding the experiments performed in ovo, the Institutional Animal Care and Use Committee, the National Institutes of Health United States, and the DIRECTIVE 2010/63/EU state that experiments on fertilized chicken eggs can be conducted without ethical approval if they are carried out up to the 14th day of development (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427727/; https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6100700/). Our experiments were performed in compliance with these conditions, on the 9th day of development.
Conflict-of-interest statement: The authors declare no conflicts of interest.
Data sharing statement: No additional data are available.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Alina Anton, Assistant Professor, Department of Toxicology, Drug Industry, Management and Legislation, “Victor Babes” University of Medicine and Pharmacy, Eftimie Murgu square nr 2, Timisoara 300041, Romania. dolghi.alina@umft.ro
Received: October 14, 2024
Revised: December 9, 2024
Accepted: January 3, 2025
Published online: February 26, 2025
Processing time: 133 Days and 16.8 Hours
Core Tip

Core Tip: Ischemic heart disease is a condition that affects a growing number of patients every day despite advances in the medical field. This phenomenon implies the need to identify new therapeutic approaches. Galium verum is a plant with cardioprotective effects, including maintenance of cardiac contractility. However, studies on Galium verum extract on cardiomyoblasts and vascular plexus have not been performed. We observed a stimulating effect of the plant on the H9C2(2-1) cell line, without signs of apoptosis or irritating effects in ovo and with a protective effect on the chorioallantoic membrane.