CD36 fatty-acid-transporter gene variants-CD36 G/A (rs1761667) and CD36 C/T (rs75326924) as biomarkers for risk-prediction in gestational diabetes mellitus

Figure 1 The polymerase chain reaction and digested products were separated on 2% and 3% agarose gels, stained with EtBr, and documented with a gel documentation system. A: 2% agarose gel showing CD36 gene products of 190 bp, amplified by polymerase chain reaction (PCR); B: 3% agarose gel showing PCR products digested with RE (HhaI), L1, L4, L6: GG (138 and 52 bp), L2 & L7: GA (190, 138 and 52 bp), L3 & L5: AA (190 bp) (M: 100 bp Ladder); C: 2% agarose gel showing CD36 gene products of 358 bp, amplified by PCR; D: 3% agarose gel showing PCR products digested with RE (Sau6I), L3 & L6: CC (358bp); L2, L5 & L7: CT (358, 243 and 114 bp); L1 & L4: TT (243 and 114 bp) (M: 100 bp Ladder).

Figure 2 CD36 gene expression in controls and gestational diabetes mellitus cases. GDM: Gestational diabetes mellitus.

Figure 3 Gene expression in gestational diabetes mellitus cases with different genotypes of CD36 rs1761667 (G/A) and rs75326924 (C/T) polymorphisms. A: CD36 rs1761667 (G/A); B: Rs75326924 (C/T). GDM: Gestational diabetes mellitus.