Copyright
©The Author(s) 2024.
World J Gastrointest Surg. Dec 27, 2024; 16(12): 3843-3856
Published online Dec 27, 2024. doi: 10.4240/wjgs.v16.i12.3843
Published online Dec 27, 2024. doi: 10.4240/wjgs.v16.i12.3843
Table 1 The scoring corresponds to hepatic morphology
| Score | Sinusoidal congestion | Cytoplasmic vacuolization | Parenchymal necrosis |
| 0 | None | None | None |
| 1 | Minimal | Minimal | Single-cell necrosis |
| 2 | Mild | Mild | < 30% |
| 3 | Moderate | Moderate | < 60% |
| 4 | Severe | Severe | > 60% |
Table 2 Gene name
| Gene name | Forward | Reverse |
| IL-1β (mouse) | 5'-GAAATGCCACCTTTTGACAGTG-3' | 5'- TGGATGCTCTCATCAGGACAG-3' |
| IL-6 (mouse) | 5'-TCCAATGCTCTCCTAACAGATAAG-3' | 5'-CAAGATGAATTGGATGGTCTTG-3' |
| TNFα (mouse) | 5'-CGTCAGCCGATTTGCTATCT-3' | 5'-CGGACTCCGCAAAGTCTAAG-3' |
| Lgr5 (mouse) | 5'-CCTACTCGAAGACTTACCCAGT-3' | 5'-GCATTGGGGTGAATGATAGCA-3' |
| NRF2 (mouse) | 5'-CATAGCAGAGCCCAGTGACG-3' | 5'-TTCCATTGTGCCTTCAGCGT-3' |
| ZNT1 (mouse) | 5'-GGAAGCGGAAGACAACAGGG-3' | 5'-CAAGGCATTCACGACCACG-3' |
| ZNT2 (mouse) | 5'-CGGAGCCCGGTCCTTCTTA-3' | 5'-GCATGGCAATAATGGTTGCTCT-3' |
| ZNT3 (mouse) | 5'-GAAGAGTCTTTTCACAGAGCCC-3' | 5'-TGTGTGCTAAATACCCACCAAC-3' |
| ZNT4 (mouse) | 5'-AAGCGCCTCAAATCCCTGC-3' | 5'-CCACCACGACTCGAAGTTTATT-3' |
| ZNT5 (mouse) | 5'-GTTGGTTTTCATACGGCTTCCA-3' | 5'-CTGAAGGCGCTTAGCTCCAC-3' |
| ZNT6 (mouse) | 5'-ATGGGGACGATTCATCTCTTTCG-3' | 5'-CACAGCACGTTGATTGCACC-3' |
| ZNT7 (mouse) | 5'-GGATGATGAATACAAACCACCCA-3' | 5'-AAAGCGAAAGAGAGGTTCAGG-3' |
| ZNT8 (mouse) | 5'-AGCCACCAAGATGTACGCC-3' | 5'-CTTGCTTGCTCGACCTGTT-3' |
| ZNT9 (mouse) | 5'-TATGTGGTTCCCGACATTCACC-3' | 5'-TGATGGGACTTTGTCAGCTTTT-3' |
| ZNT10 (mouse) | 5'-GGCCGTTACTCAGGCAAGAC-3' | 5'-GCATGTTGAACGAGTCCGAGA-3' |
| ZIP1 (mouse) | 5'-ACCGAGTTATCACAGCCACC-3' | 5'-TGGTTAGCCCTGACCTTCG-3' |
| ZIP2 (mouse) | 5'-AGGAGTAAAAATCGGCTGCCT-3' | 5'-TGTAGCTGCATCCATCTGGAAC-3' |
| ZIP3 (mouse) | 5'-TGTAGCCAGGAATACGCCAC-3' | 5'-TGCCTGTGAAGGTCATCGAG-3' |
| ZIP4 (mouse) | 5'-CGGGCCACCAGCGTATTTA-3' | 5'-AGGGACTTGTGTTGGCTCTG-3' |
| ZIP5 (mouse) | 5'-ATGGTCAGCCAACGAATGGT-3' | 5'-TGGACATCTGGGCAGGAATG-3' |
| ZIP6 (mouse) | 5'-TGGGCGAGATCCTTTCCCTA-3' | 5'-CAGTCACACGGTTGCTGGTA-3' |
| ZIP7 (mouse) | 5'-GACAGTGTCCAGGTGGTGTT-3' | 5'-CATGGCCACTCCCACGATAG-3' |
| ZIP8 (mouse) | 5'-AACCAGCTCGAACTTCTCTGC-3' | 5'-GAAAGACTGGGCTTTGCGTTG-3' |
| ZIP9 (mouse) | 5'-GCAGTTCCAAAATCACCACCA-3' | 5'-TTGCCCAAGAGCATTCAGTGT-3' |
| ZIP10 (mouse) | 5'-TCACTGTGAGCAACGGAGTC-3' | 5'-GCTGAACTGCCCATCAAAGC-3' |
| ZIP11 (mouse) | 5'-CTGCCCGCTGGAGAATATGA-3' | 5'-CAAGGTTACAGCTCCGTGGT-3' |
| ZIP12 (mouse) | 5'-TCCTCTACTGAGACCGGCAA-3' | 5'-CAGTGGTCACCAGCAGAGAG-3' |
| ZIP13 (mouse) | 5'- CTGCCTGTCGCCTGGATAAT-3' | 5'-CAAGGGGAAAACCCCACTGA-3' |
| ZIP14 (mouse) | 5'-TCTGGTTTTCTGAGGTGCAGG-3' | 5'-GCTCTGGAGACCTCTTTGCG-3' |
| ACTIN (mouse) | 5'-GGCTGTATTCCCCTCCATCG-3' | 5'-CCAGTTGGTAACAATGCCATGT-3' |
- Citation: Cheng MZ, Luo JH, Li X, Liu FY, Zhou WJ. Zinc pretreatment for protection against intestinal ischemia-reperfusion injury. World J Gastrointest Surg 2024; 16(12): 3843-3856
- URL: https://www.wjgnet.com/1948-9366/full/v16/i12/3843.htm
- DOI: https://dx.doi.org/10.4240/wjgs.v16.i12.3843