Sun QY, Cheng YM, Sun YH, Huang J. New rabbit model for benign biliary stricture formation with repeatable administration. World J Gastrointest Surg 2024; 16(11): 3538-3545 [DOI: 10.4240/wjgs.v16.i11.3538]
Corresponding Author of This Article
Jin Huang, MD, PhD, Chief Doctor, Professor, Department of Gastroenterology, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, No. 68 Gehu Road, Wujin District, Changzhou 213161, Jiangsu Province, China. hj042153@hotmail.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastrointest Surg. Nov 27, 2024; 16(11): 3538-3545 Published online Nov 27, 2024. doi: 10.4240/wjgs.v16.i11.3538
New rabbit model for benign biliary stricture formation with repeatable administration
Qiu-Yue Sun, Yi-Ming Cheng, Yu-Hui Sun, Jin Huang
Qiu-Yue Sun, Yi-Ming Cheng, Yu-Hui Sun, Jin Huang, Department of Gastroenterology, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou 213161, Jiangsu Province, China
Qiu-Yue Sun, Yu-Hui Sun, Department of Gastroenterology, Graduate School of Nanjing Medical University, Nanjing 211166, Jiangsu Province, China
Author contributions: Sun QY wrote the paper, performed the experiments, and acquired the data; Cheng YM analyzed and interpreted the data; Sun YH contributed to the linguistic formatting and correction of the manuscript; Huang J designed the research; all authors have read and approved the final manuscript.
Supported byThe Key Project of Changzhou Medical Center of Nanjing Medical University, No. CMCM202310 and No. CMCC202209; and Science and Technology Development Fund of Nanjing Medical University, No. NMUB20220196.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Ethics Committee of Changzhou University.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The data are available from the corresponding author at hj042153@hotmail.com.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Jin Huang, MD, PhD, Chief Doctor, Professor, Department of Gastroenterology, The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, No. 68 Gehu Road, Wujin District, Changzhou 213161, Jiangsu Province, China. hj042153@hotmail.com
Received: June 19, 2024 Revised: August 29, 2024 Accepted: September 10, 2024 Published online: November 27, 2024 Processing time: 132 Days and 21 Hours
Abstract
BACKGROUND
The treatment of benign biliary strictures (BBS) is a challenging clinical problem. At present, there is a lack of ideal models for the study of BBS treatment.
AIM
To develop a novel animal model of BBS to simulate studies on the processes and mechanisms in the human condition.
METHODS
A rabbit model of benign bile duct stricture was established by surgical injury of the bile duct. After removal of the gallbladder, a drainage tube was placed through the cystic duct at the stump, and a BBS model was induced by surgical injury at the lower end of the common bile duct.
RESULTS
Compared with the control group, the model rabbits showed gross jaundice, increased serum bilirubin, and decreased liver function. Cholangiography showed segmental bile duct stenosis in the model rabbits. Pathological staining showed inflammatory cell infiltration and fibrosis in the biliary tract of rabbits in the model group. This was consistent with the clinical manifestations of BBS. This model provided serology, imaging, pathology, and other aspects of BBS.
CONCLUSION
We have successfully established an animal model of benign stricture of the lower bile duct with repeatable administration, which is consistent with the clinical manifestations of BBS.
Core Tip: A model of benign biliary stricture (BBS) was established and certified by biliary drainage angiography, pathology, and other evaluations. This model could allow better long-term low-dose repeated action of drugs on biliary scars, and provide new ideas for the treatment of BBS. When the efficacy of this model is confirmed, new biological agents can be used to treat BBS in humans through endoscopic retrograde cholangiopancreatography in the future.
