Fractional carbon dioxide laser-induced photothermal activation of mesenchymal stem cell-derived exosomes accelerates diabetic wound healing by enhancing angiogenesis

Table 1 The sequences of sphingosine-1-phosphate receptor 1 shRNA

ID	Sequence (5’-3’)
shS1PR1 1	GCGGGAAGGGAGTATGTTTGTCTCGAGACAAACATACTCCCTTCCCGC
shS1PR1 2	GCAATAACTTCCGCCTCTTCCCTCGAGGGAAGAGGCGGAAGTTATTGC
shS1PR1 3	GGATCATGTCCTGCTGCAACTCGAGTTGCAGCAGGACATGATCC

shS1PR1: Sphingosine-1-phosphate receptor 1 shRNA.

Table 2 Primers used in the study

Primer	Specie	Forward (5’-3’)	Reverse (5’-3’)
S1PR1	Human	TCTGCGGGAAGGGAGTATGTT	GCAGGAAGAGGCGGAAGTTATT
S1PR2	Human	CGTCTTTATCGTCTGCTGGCTG	TAGTGGGCTTTGTAGAGGATCG
S1PR3	Human	CCTCTACGCACGCATCTACTTC	AACACGCTCACCACAATCACC
GAPDH	Human	GGAAGCTTGTCATCAATGGAAATC	TGATGACCCTTTTGGCTCCC

S1PR: Sphingosine-1-phosphate receptor; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

Table 3 Primary antibodies used in the study

Antibody	Company	Catalog number	Dilution ratio
Anti-CD63	HuaBio	CatET1607-2	1:2000
Anti-TSG101	HuaBio	CatET1701-59	1:2000
Anti-HSP90	Proteintech	Cat13171-1-AP	1:2000
Anti-phospho-AKT	Proteintech	Cat28731-1-AP	1:2000
Anti-AKT	Proteintech	Cat10176-2-AP	1:2000
Anti-HIF-1α	Cell signaling	Cat36169	1:1000
Anti-VEGFA	Proteintech	Cat19003-1-AP	1:1000
Anti-S1PR1	Proteintech	Cat55133-1-AP	1:1000
Anti-S1PR2	Proteintech	Cat21180-1-AP	1:1000
Anti-S1PR3	Proteintech	Cat84697-1-RR	1:5000
Anti-β-actin	Proteintech	Cat66009-1-Ig	1:10000

TSG101: Tumor susceptibility gene 101; HSP90: Heat shock protein 90; Phospho-AKT: Phosphorylated protein kinase B; AKT: Protein kinase B; HIF-1α: Hypoxia-inducible factor 1 alpha; VEGFA: Vascular endothelial growth factor A; S1PR: Sphingosine-1-phosphate receptor.