Effect of fish scale ointment on diabetic foot ulcer by inducing ferroptosis via the nuclear factor E2-related factor 2 pathway

Figure 1 Hematoxylin and eosin staining of the new blood vessels in rat wound tissue. FSC: Fish scale collagen; Lip-1: Liproxstatin-1.

Figure 2 Immunohistochemistry staining of basic fibroblast growth factor and CD31 protein. FSC: Fish scale collagen; Lip-1: Liproxstatin-1; bFGF: Basic fibroblast growth factor.

Figure 3 Levels of ferroptosis-related proteins in rat wound tissues. A: Western blot bar plot of the proteins; B: Nuclear factor E2-related factor 2 protein expression level; C: Heme oxygenase-1 protein expression level; D: Glutathione peroxidase 4 protein expression level. ^aP < 0.05 vs control; ^bP < 0.05 vs model; ^cP < 0.05 vs fish scale collagen. GPX4: Glutathione peroxidase 4; HO-1: Heme oxygenase-1; Nrf2: Nuclear factor E2-related factor 2; FSC: Fish scale collagen; Lip-1: Liproxstatin-1.