Huang TT, Sun WJ, Liu HY, Ma HL, Cui BX. p66Shc-mediated oxidative stress is involved in gestational diabetes mellitus. World J Diabetes 2021; 12(11): 1894-1907 [PMID: 34888014 DOI: 10.4239/wjd.v12.i11.1894]
Corresponding Author of This Article
Bao-Xia Cui, PhD, Chief Physician, Professor, Department of Obstetrics and Gynecology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, No. 107 Wenhua Xi Road, Jinan 250013, Shandong Province, China. cuibaoxia@sdu.edu.cn
Research Domain of This Article
Obstetrics & Gynecology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Diabetes. Nov 15, 2021; 12(11): 1894-1907 Published online Nov 15, 2021. doi: 10.4239/wjd.v12.i11.1894
p66Shc-mediated oxidative stress is involved in gestational diabetes mellitus
Ting-Ting Huang, Wen-Juan Sun, Hai-Ying Liu, Hong-Li Ma, Bao-Xia Cui
Ting-Ting Huang, Cheeloo College of Medicine, Shandong University, Jinan 250000, Shandong Province, China
Ting-Ting Huang, Hong-Li Ma, Department of Obstetrics, Taian City Central Hospital, Taian 271000, Shandong Province, China
Wen-Juan Sun, Department of Obstetrics, The Second Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250000, Shandong Province, China
Hai-Ying Liu, Department of Obstetrics and Gynecology, Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, Qingdao 266000, Shandong Province, China
Bao-Xia Cui, Department of Obstetrics and Gynecology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, Jinan 250013, Shandong Province, China
Author contributions: Huang TT, Sun WJ, Liu HY, and Cui BX designed and coordinated the study; Huang TT, Sun WJ, and Ma HL performed the experiments, acquired, and analyzed the data; Huang TT, Sun WJ, and Liu HY interpreted the data; Huang TT and Sun WJ wrote the manuscript; All authors approved the final version of the article.
Supported byThe Scientific Research Fund of Qilu Hospital (Qingdao), No. QDKY2015ZD04.
Institutional review board statement: The study was reviewed and approved by the Taian City Central Hospital Institutional Review Board (Approval NO. 2016-05-15).
Conflict-of-interest statement: The authors declare that they have no conflicting interests.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at cuibaoxia@sdu.edu.cn. Participants gave informed consent for data sharing.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Bao-Xia Cui, PhD, Chief Physician, Professor, Department of Obstetrics and Gynecology, Qilu Hospital, Cheeloo College of Medicine, Shandong University, No. 107 Wenhua Xi Road, Jinan 250013, Shandong Province, China. cuibaoxia@sdu.edu.cn
Received: July 22, 2021 Peer-review started: July 22, 2021 First decision: August 16, 2021 Revised: August 29, 2021 Accepted: September 19, 2021 Article in press: September 19, 2021 Published online: November 15, 2021 Processing time: 116 Days and 5.6 Hours
ARTICLE HIGHLIGHTS
Research background
Oxidative stress and mitochondrial dysfunction in the placenta are closely related to the onset of gestational diabetes mellitus (GDM). p66Shc plays a role in regulating mitochondrial oxidative stress, and dynamin-related protein 1 (Drp1) is a necessary dynamic protein for mitosis of primarily localized mitochondria.
Research motivation
The motivation was to add to what is known of the role of placental mitochondria in the etiology of GDM.
Research objectives
The study aimed to investigate the potential mechanism of p66Shc in GDM.
Research methods
We detected the expression of Drp1 and p66Shc in patients with GDM and investigated the possible pathogenesis of GDM through in vitro culture of the JEG3 human trophoblast line.
Research results
P66Shc, Drp1, and reactive oxygen species (ROS) were highly expressed in the placentas and peripheral blood during GDM and in JEG3 cells under high glucose conditions. A significant increase in the expression of Drp1 and the level of ROS was detected in JEG3 cells overexpressing activated p66Shc. In contrast, p66Shc knockdown reduced the expression of Drp1 and the level of ROS.
Research conclusions
The increased expression of p66Shc induced by high glucose-activated Drp1 and promoted ROS overproduction, which may contribute to the occurrence and development of GDM.
Research perspectives
This study may provide a new understanding of molecular mechanism and experimental basis for the role of mitochondrial damage in the pathogenesis of gestational diabetes mellitus and provide a new approach for the treatment of the condition and its complications.
