Uncommon hepatocellular carcinoma in a non-cirrhotic woman with chronic hepatitis B virus infection after medical assisted reproduction: A case report

doi:10.4251/wjgo.v18.i4.116955

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Apr 15, 2026 (publication date) through Apr 11, 2026

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World Journal of Gastrointestinal Oncology

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1948-5204

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Gastrointest Oncol. Apr 15, 2026; 18(4): 116955
Published online Apr 15, 2026. doi: 10.4251/wjgo.v18.i4.116955

Table 1 Forward and reverse primers used for the quantification of hepatitis B virus DNA, hepatitis B virus covalently closed DNA and hepatitis B virus RNA in tumour and non-tumour tissues

Name	Polarity	Sequence primer (5’ to 3’)	Position¹
HBVDNAfwd	Forward	CTCGTGGTGGACTTCTCTC	255-273
HBVDNArev	Reverse	CAGCAGGATGAAGAGGAA	420-403
HBVDNAProbe	Reverse	6FAM-AACCAGGACAAATTGGAGGACAGGA-TMR	370-346
cccDNAfwd	Forward	CTCCCCGTCTGTGCCTTCT	1547-1565
cccDNArev	Reverse	GCCCCAAAGCCACCCAAG	1902-1885
cccDNAProbe	Reverse	6FAM-CATGCGACGTGCAGAGGTGAAGCGAA-TMR	1612-1587
HBVRNAfwd	Forward	CTCGTGGTGGACTTCTCTC	255-273
HBVRNArev	Reverse	CAGCAGGATGAAGAGGAA	420-403
HBVRNAProbe	Reverse	6FAM-AACCAGGACAAATTGGAGGACAGGA-TMR	370-346

¹Nucleotide positions of primers are numbered from the EcoRI site, following the GenBank sequence NC_003977.2.

HBV: Hepatitis B virus; cccDNA: Covalently closed circular DNA.

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Citation: Asero C, Ciappina G, Franzè MS, Maltese T, Gruttadauria S, Pagano D, Lombardo D, Pitrone A, Musolino C, Berretta M, Cacciola I. Uncommon hepatocellular carcinoma in a non-cirrhotic woman with chronic hepatitis B virus infection after medical assisted reproduction: A case report. World J Gastrointest Oncol 2026; 18(4): 116955
URL: https://www.wjgnet.com/1948-5204/full/v18/i4/116955.htm
DOI: https://dx.doi.org/10.4251/wjgo.v18.i4.116955