Regulator of chromosome condensation 1 promotes hepatocellular carcinoma proliferation via cell-division-cycle-associated-8 dependent phosphoinositide 3-kinase/protein kinase B signaling

Table 1 Sequences of small interfering RNAs

siRNAs	Sense/antisense	Sequences
siRCC1	Sense	5’-GGCACAGAAUCUUGCUUCAUATT-3’
	Antisense	5’-UAUGAAGCAAGAUUCUGUGCCTT-3’
siCDCA8	Sense	5’-GGAAAUACGAAUCAAGCAATT-3’
	Antisense	5’-UUGCUUGAUUCGUAUUUCCTT-3’

siRNA: Small interfering RNA; siRCC1: Small interfering regulator of chromosome condensation 1; siCDCA8: Small interfering cell division cycle-associated 8.

Table 2 Primer sequences used in real time quantitative reverse transcription polymerase chain reaction

Gene symbol	Forward/reverse	Sequences
RCC1	Forward	5’-ACTCCGGGCACAGAATCTTG-3’
	Reverse	5’-CCTGGAGATGAGGGTGGGTA-3’
CDCA8	Forward	5’-GAAGCTCGCCTCCTTTCTGA-3’
	Reverse	5’-CTCTTCCAGGGCCTGTTTGT-3’
MCM4	Forward	5’-CCTGGTCGCACTGTACTACC-3’
	Reverse	5’-AAACCATTCCCCGGCTACTG-3’
CCNB1	Forward	5’-TGTGTGCCCAAGAAGATGCT-3’
	Reverse	5’-AAGTGCAAAGGTAGAGGCCG-3’
CDCA5	Forward	5’-CTCTGAACTCCCGAGCATCC-3’
	Reverse	5’-GATTGGACAGCTGGGACCTC-3’
GAPDH	Forward	5’-CCAGAACATCATCCCTGCCT -3’
	Reverse	5’-CCTGCTTCACCACCTTCTTG -3’