Identifying adipocyte-derived exosomal miRNAs as potential novel prognostic markers for radiotherapy of esophageal squamous cell carcinoma

Table 1 Clinical characteristics of patients with esophageal squamous cell carcinoma

Characteristics	ESCC, n
Sex
Male	33
Female	67
Age
< 60 years	21
≥ 60 years	79
Length
< 5 cm	54
≥ 5 cm	46
Therapeutic modality
Radiotherapy	58
Chemoradiotherapy	42
T stage
T1–2	52
T3–4	48
Lymph node metastasis
N0–1	93
N2–3	7
TNM stage
I-II	75
III-IV	25

TNM: Tumor-node-metastasis; ESCC: Esophageal squamous cell carcinoma.

Table 2 List of primer sequences of related RNAs

Primer	Sequences (5' to 3')
hsa-miR-660-5p-F	CGCGGCATACCCATTGCATATC
hsa-miR-660-5p-R	ATCCAGTGCAGGGTCCGAGG
hsa-miR-660-5p-RT	GTCGTATCCAGTGCAGGGTCCGAGGTAT TCGCACTGGATACGACCAACTC
hsa-miR-378a-3p-F	AATCCGGAACTGGACTTGGAGTC
hsa-miR-378a-3p-R	ATCCAGTGCAGGGTCCGAGG
hsa-miR-378a-3p-RT	GTCGTATCCAGTGCAGGGTCCGAGGTAT TCGCACTGGATACGACGCCTTC
hsa-miR-193b-3p-F	AACACGCAACTGGCCCTCAAA
hsa-miR-193b-3p-R	ATCCAGTGCAGGGTCCGAGG
hsa-miR-193b-3p-RT	GTCGTATCCAGTGCAGGGTCCGAGGTAT TCGCACTGGATACGACAGCGGG
hsa-mir-30a-F	CCGCTCGTGTAAACATCCTCGA
hsa-mir-30a-R	ATCCAGTGCAGGGTCCGAGG
hsa-mir-30a-RT	GTCGTATCCAGTGCAGGGTCCGAGGTAT TCGCACTGGATACGACCTTCCA

Table 3 The D0, N, and SER values of cells subjected to different treatment conditions

Cell	Group	N	Dq	D0	SER
KYSE-30	Control	1.710	1.105	2.060	0.832
	+ Adipocyte	1.615	1.186	2.475
	Control	1.337	0.705	2.426	0.883
	+ Exosome	1.513	1.137	2.746
TE-1	Control	1.395	0.745	2.239	0.825
	+ Adipocyte	1.248	0.601	2.713
	Control	1.724	1.022	1.876	0.751
	+ Exosome	1.885	1.584	2.498

Sensitizer enhancement ratios value was simulated using the multi-target single-hit model. D₀: Mean lethal dose; N: Extrapolation number; SER: Sensitizer enhancement ratios.