Transient receptor potential melastatin 6 and transient receptor potential melastatin 6/7 antagonists suppress colon adenocarcinoma HT-29 cells

Table 1 Phosphorylated residue of transient receptor potential melastatin 6 proteins

	Parental HT-29	Spheroid-derived HT-29
N-terminus	S12, S67, S78, Y80, T87, T88, S90, T92, T94, S141, T169, T170, T176, T181, Y228, S243, S246, S274, S282, S302, T380, S386, T398, S407, Y431, S445, Y462, Y479, T481, T509, T530, S552, S558, Y574, S586, S596, T602, Y608, T635, S662, Y697, S705, T706, S743, T756, S820, Y835, Y845, T859, S869	S12, S32, S78, Y80, T87, T88, T94, Y111, Y116, S141, S153, T169, T170, T176, T181, S184, T204, Y228, T230, S236, T239, Y259, S274, S282, T354, S362, T380, S407, S409, Y431, S445, T471, Y506, T509, Y519, Y525, Y529, T530, Y545, S551, S552, S558, Y574, S586, S590, T602, Y645, S655, Y668, S669, S705, T706, S713, S743, T759, S769, S774, S784, S789, S790, S791, S794, S796, S820, Y845, S869 (65)
Channels	T881, S893, T899, T936, S951, T974, S1000, S1006, Y1022, S1038, T1046, Y1053	S876, T881, S893, S907, T913, T915, Y966, T968, T974, Y979, S1008, Y1026, Y1053
TRP	Y1073, S1078, Y1086, Y1089, T1094, Y1095, S1109	Y1073, S1078, S1080, Y1086, Y1089, Y1091, T1094, Y1095
C-terminus	Y1137, S1139, Y1157, S1168, T1231, S1281, S1285, T1322, S1324, S1329, S1349, S1357, T1375, T1391, S1395, S1399, S1736, S1737, T1739, Y1741, S1746, S1747, S1986, T1993, T2011	Y1137, S1139, Y1157, S1168, T1231, S1244, T1245, S1252, S1254, Y1273, S1277, S1281, S1285, T1311, S1332, S1339, Y1341, S1368, T1375, S1736, S1737, T1739, Y1741, S1746, S1747, S1970, Y1984, T1992, S2002, T2011, S2015
Coiled coil	T1176, S1177, T1181, Y1184, S1195, S1200, S1206, T1221, S1226	T1176, S1177, T1181, Y1184, S1195, S1200, S1206, T1221, S1226
S/T rich domain	S1438, S1441, S1458, T1463, S1487, S1498, S1500, S1503, Y1533, S1539, S1541, T1577, T1598, S1603, S1616, S1618, S1623, T1628, S1630, S1633, T1635, T1647, Y1660, S1672, T1679, S1685, S1689, S1690, S1697, S1699, S1702, T1739, Y1741	T1435, S1437, S1467, T1474, S1478, T1479, S1498, S1500, T1523, Y1533, S1539, S1541, S1562, S1563, T1577, S1583, T1589, S1605, Y1622, S1623, T1628, S1630, S1658, S1664, S1669, S1678, T1679, S1685, S1689, S1690, S1697, S1699, S1702, T1739, Y1741
Dimerization motif	Y1710, S1711, S1722, T1724, T1728, Y1731	Y1710, S1711, S1722, T1724, T1728, Y1731
α-kinase domain	S1754, S1757, S1821, T1822, T1843, Y1854, Y1878, Y1880, T1895, T1897, S1908, Y1912, Y1914, T1915, T1932, S1935	S1754, S1759, S1771, S1787, S1790, T1813, T1822, T1843, Y1854, T1855, Y1865, S1868, Y1886, S1908, Y1912, Y1914, T1915, S1944

TRP: Transient receptor potential.

Table 2 Phosphorylated residue of transient receptor potential melastatin 7 proteins

	Parental HT-29	Spheroid-derived HT-29
N-terminus	S9, T10, T12, Y18, S22, S23, T55, S57, Y62, S79, T84, S87, T89, S101, S103, Y104, Y108, S113, T115, S138, S193, S196, S233, S243, Y256, T270, Y303, T318, Y327, T332, T349, T353, S359, T379, S385, T397, S406, Y430, S453, S464, T470, Y505, T508, Y528, S539, S547, S552, S554, S561, T564, T583, Y587, T603, T615, S648, S683, T737, T739, S745, T778, S788, T807 S823, S824, S836, Y849	S2, S9, T10, Y18, S22, S23, T55, S57, Y62, S63, S74, S79, S87, T89, Y92, S101, S103, Y108, S112, Y113, T115, S138, T166, T167, T173, S195, S196, T201, Y225, T227, S243, T252, T269, T318, T332, T353, S359, T367, T379, Y430, S438, S453, S464, T508, S539, S547, S552, S553, S554 T555, S561, T564, T583, Y587, T603, T615, S648, S661, S676, S727, S728, T778, S783, S788, T795, S799, S823, S824, S836, Y849, T860, Y863
Channels	T895, Y896, S913 S921, S927, T929, S934, Y965, Y989, Y1023, S1031, Y1051, S1060, T1064, T1070, T1073, Y1080, Y1085	T895, Y896, S913, S921, S927, S929, Y949, Y953, Y989, Y1002, S1013, Y1023, S1029, S1031, Y1041, Y1051, S1060, T1064, Y1080, Y1085
TRP	Y1100, S1107, Y016, Y1122, S1036, S1040, S1141	Y1100, S1107, Y016, Y1122, S1036, S1040, S1141
C-terminus	T1154, S1155, T1163, Y1181, S1191, S12551, S1258, T1265, S1269, S1271, S1292, T1296, S1298, S12991, S13001, S1308, S13491, S1350, S13601, S1587, S1888, S1590, Y1826, T18271, S1839, S1848, T1849, T1855	T1154, S1155, T1163, Y1181, S1191, S1193, S12551, S1258, T1265, S1269, S1271, S1292, T1296, S1298, S12991, S13001, S1308, S13491, S1350, S1357, S13601, T1580, S1587, S1588, S1590, Y1826, T18271, S1839, S1848, T1849, S1852, T1855, S1857
Coiled coil	T1200, S1208, S12241, S1227, T1242, T1245, T1248, T12501	T1200, Y1220, S12241, S1227, S1230, S1239, T1242, T12501
S/T rich domain	S13861, S1389, S13901, S1394, S13951, S14031, T1404, S1406, S1409, S1412, S14161, T1418, S14451, S1455, S1463, T14661, T1470, Y1479, T1485, T14871, S14881, S14911, T1493, S14951, S14971, S15011, T15021, S15051, S15101, T1524, S1530, T1534, S15401, T1548	S13861, S1389, S13901, S1394, S13951, S14031, T1404, S1406, S1409, S1412, S14161, T1418, Y1426, S14451, T1454, S1455, S1463, T14661, T1470, Y1479, T1485, T14871, S14881, S14911, T1493, S14951, S14971, S15011, T15021, S15051, S15101, T1524, S1530, T1534, S15401, T1548
Dimerization motif	S1553, S15641, S15661	S1553, S15641, S15661
α-kinase domain	S1595, S1597, S1598, S1600, S1612, T1629, S1631, Y1642, S1656, S1657, Y1659, T1663, S16921, Y1695, Y1696, S1709, Y1727, T1738, T1740, S1749, T1756, S1776, S1785	S1592, S1597, S1598, S1600, S1612, T1629, S1638, S1646, S1656, Y1659, T1663, T1682, S16921, Y1695, Y1696, S1709, Y1727, T1738, T1740, S1749, T1752, Y1753, Y1755, T1756, S1776, S1782, S1811

¹Phosphorylated residues modified by the α-kinase domain of transient receptor potential melastatin 6.

TRP: Transient receptor potential.

Table 3 Methionine oxidation in transient receptor potential melastatin 6 and transient receptor potential melastatin 7 proteins

TRPM6		TRPM7
Parental HT-29	Spheroid-derived HT-29	Parental HT-29	Spheroid-derived HT-29
M33, M63, M133, M338, M370, M416, M618, M623, M625, M648, M657, M732, M768, M847, M864, M969, M984, M1020, 1061, M1076, M1093, M1162, M1278, M1183, M1434, M1436, M1575, M1879, M2020	M1, M33, M133, M244, M338, M370, M416, M444, M450, M618, M623, M625, M648, M657, M727, M734, M739, M768, M847, M969, M984, M1093, M1162, M1190, M1265, M1434, M1436, M1575, M1719, M1879, M1904, M1947, M2020	M43, M372, M632, M637, M649, M706, M742, M746, M782, M794, M812, M868, M878, M906 M991, M996, M1000, M1088, M1020, M1180, M1207, M1287, M1319, M1373, M1446, M1616, M1720, M1745	M130, M575, M591, M595, M649, M662, M704, M706, M741, M746, M773, M782, M812, M868, M991, M992, M996, M1000, M1180, M1207, M1287, M1318, M1373, M1446, M1528, M1561, M1616, M1720, M1745, M1788

TRPM: Transient receptor potential melastatin.