Effectiveness of multi-region 16S rRNA gene sequencing in studying the microbiome of gastric cancer tissues

Table 1 Comparison of detection rates between multi- and single-region 16S rRNA gene sequencing in different microbial abundances under positive control

Group	n	Bacterial concentration
		103 CFU/mg	102 CFU/mg	10 CFU/mg
Multi-region sequencing	59	92.86 ± 3.52	76.43 ± 5.15	34.24 ± 4.87
Single-region sequencing	59	45.65 ± 6.27	18.96 ± 4.74	2.38 ± 1.19
t value	-	50.413	67.219	45.681
P value	-	< 0.001	< 0.001	< 0.001

Table 2 Comparison of operational taxonomic unit numbers detected using multi- and single-region 16S rRNA gene sequencing in gastric cancer tissues

Group	n	Genus		Species
		Paraffinized tissue	Fresh tissue	Paraffinized tissue	Fresh tissue
Multi-region sequencing	59	2.67 ± 0.58	2.33 ± 1.53	1.03 ± 0.35	0.67 ± 0.18
Single-region sequencing	59	74.21 ± 7.81	51.00 ± 16.09	32.33 ± 5.51	28.67 ± 9.07
t value	-	70.224	25.392	46.861	25.937
P value	-	< 0.001	< 0.001	< 0.001	< 0.001

Table 3 Comparison of microbial group alpha diversity using multi- and single-region 16S rRNA gene sequencing in gastric cancer tissues

Group	n	Shannon		Simpson		Chao1
		Paraffinized tissue	Fresh tissue	Paraffinized tissue	Fresh tissue	Paraffinized tissue	Fresh tissue
Multi-region sequencing	59	1.18 ± 0.12	0.68 ± 0.18	0.63 ± 0.08	0.37 ± 0.07	14.67 ± 3.21	3.33 ± 3.21
Single-region sequencing	59	3.08 ± 0.39	2.88 ± 0.40	0.89 ± 0.15	0.88 ± 0.24	199.67 ± 22.50	115.83 ± 31.95
t value	-	35.672	38.917	12.621	15.804	63.194	25.071
P value	-	< 0.001	< 0.001	< 0.001	< 0.001	< 0.001	< 0.001