©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Oct 15, 2025; 17(10): 110997
Published online Oct 15, 2025. doi: 10.4251/wjgo.v17.i10.110997
Published online Oct 15, 2025. doi: 10.4251/wjgo.v17.i10.110997
Effectiveness of multi-region 16S rRNA gene sequencing in studying the microbiome of gastric cancer tissues
Ting-Ting Wu, Xiang Zhou, Qi Huang, Qi Yang, Clinical Laboratory, Liyang People's Hospital, Changzhou 213300, Jiangsu Province, China
Author contributions: Wu TT designed the research study and contributed new reagents and analytical tools; Wu TT performed the research; Zhou X, Huang Q, and Yang Q analyzed the data and wrote the manuscript; all authors have read and approved the final version of the manuscript.
Supported by Liyang People's Hospital Technology Project, No. 2021YJKT0013.
Institutional review board statement: This study was approved by the Medical Ethics Committee of Liyang People's Hospital (approval No. 2025045).
Informed consent statement: All study participants or their legal guardians provided written informed consent before enrollment.
Conflict-of-interest statement: The authors report no relevant conflicts of interest.
Data sharing statement: No additional data are available.
Corresponding author: Ting-Ting Wu, Deputy Director, Clinical Laboratory, Liyang People's Hospital, No. 70 Jianshe West Road, Licheng Town, Liyang, Changzhou 213300, Jiangsu Province, China. wtt15335009416@163.com
Received: July 11, 2025
Revised: August 9, 2025
Accepted: September 10, 2025
Published online: October 15, 2025
Processing time: 94 Days and 22.9 Hours
Revised: August 9, 2025
Accepted: September 10, 2025
Published online: October 15, 2025
Processing time: 94 Days and 22.9 Hours
Core Tip
Core Tip: This study demonstrated that multi-region 16S rRNA gene sequencing provides significantly improved species resolution and detection rates compared with traditional single-region method. In both positive controls and gastric cancer tissue samples, it revealed richer and more diverse microbial communities. These findings suggest that compared to single-region sequencing, multi-region sequencing is a more accurate and effective approach for profiling the microbiome in gastric cancer research.