Sm-like 5 knockdown inhibits proliferation and promotes apoptosis of colon cancer cells by upregulating p53, CDKN1A and TNFRSF10B

doi:10.4251/wjgo.v16.i6.2716

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World Journal of Gastrointestinal Oncology

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1948-5204

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastrointest Oncol. Jun 15, 2024; 16(6): 2716-2726
Published online Jun 15, 2024. doi: 10.4251/wjgo.v16.i6.2716

Table 1 The primer sequences used for real-time quantitative polymerase chain reaction

Genes	Upstream	Downstream	Size (bp)
GAPDH	TGACTTCAACAGCGACACCCA	CACCCTGTTGCTGTAGCCAAA	121
LSM5	CTAACGCTACTACCAACCCGT	TCCTTCTCCTCCAGGAACCAG	257
CDK1	GGATGTGCTTATGCAGGATTCC	CATGTACTGACCAGGAGGGATAG	100
MDM2	GAATCATCGGACTCAGGTACATC	TCTGTCTCACTAATTGCTCTCCT	167
CCNE2	GGAACCACAGATGAGGTCCAT	CCATCAGTGACGTAAGCAAACT	237
RALB	TCATCAGGAAAGGAGCACT	GAGGGGATACAGGATTGTT	189
CDKN1A	CTGTCACTGTCTTGTACCCTTGT	AAATCTGTCATGCTGGTCTGC	113
TNFRSF10B	GCAGACTTGGTGCCCTTTG	CCCACTTTATCAGCATCGTGT	133
AKT2	GCGGAAGGAAGTCATCATTG	GTGGGTCTGGAAGGCATAC	118
TP53	CCTCCTCAGCATCTTATCC	ACAAACACGCACCTCAAA	258
IRF1	GATGCTTCCACCTCTCACC	TGCTCCACCTCCAAGTCC	201

LSM5: Sm-like 5; CDKN1A: Cyclin-dependent kinase inhibitor 1A; TNFRSF10B: Tumor necrosis factor receptor superfamily 10B.

Full Size Table

Table 2 RNA quality inspection results

Group	Sample name	Thermo NanoDrop 2000		2100 Result		Quality inspection results
Group	Sample name	Concentration (ng/μL)	A260/A280	RIN	28S/18S
ShCtrl	6849-1	663.7	1.98	10	2.1	Qualified
	6849-2	663.7	1.98	10	2.5	Qualified
	6849-3	663.7	1.98	10	2.2	Qualified
ShLSM5	6850-1	418.1	1.91	9.7	1.2	Qualified
	6850-2	418.1	1.91	9.8	1.1	Qualified
	6850-3	418.1	1.91	9.5	1.2	Qualified

LSM5: Sm-like 5.

Full Size Table

Citation: Mo CJ, Deng XY, Ma RL, Zhu K, Shi L, Li K. Sm-like 5 knockdown inhibits proliferation and promotes apoptosis of colon cancer cells by upregulating p53, CDKN1A and TNFRSF10B. World J Gastrointest Oncol 2024; 16(6): 2716-2726
URL: https://www.wjgnet.com/1948-5204/full/v16/i6/2716.htm
DOI: https://dx.doi.org/10.4251/wjgo.v16.i6.2716