Impact of Alcian blue and periodic acid Schiff expression on the prognosis of gastric signet ring cell carcinoma

Table 1 Correlation between periodic acid Schiff expression and clinicopathologic features (n = 83), n (%)

Factors	Total	PAS		P value
	n = 83	≤ 50% positive	> 50% positive
Age (yr)				0.583
≤ 60	63 (75.9)	24 (72.7)	39 (78.0)
> 60	20 (24.1)	9 (27.3)	11 (22.0)
Gender				0.898
Male	32 (38.6)	13 (39.4)	19 (38.0)
Female	51 (61.4)	20 (60.6)	31 (62.0)
Tumor diameter				0.347
≤ 3	43 (51.8)	15 (45.5)	28 (56.0)
> 3	40 (48.2)	18 (54.5)	22 (44.0)
Vessel carcinoma embolus				0.608
No	33 (39.8)	12 (36.4)	21 (42.0)
Yes	50 (60.2)	21 (63.6)	29 (58.0)
Lymph node metastasis				0.331
Negative	33 (39.8)	11 (33.3)	22 (44.0)
Positive	50 (60.2)	22 (66.7)	28 (56.0)
Tumor stage				0.006
Early	21 (25.3)	3 (9.1)	18 (36.0)
Advanced	62 (74.7)	30 (90.9)	32 (64.0)
Ki67				0.394
≤ 50%	62 (74.7)	23 (69.7)	39 (78.0)
> 50%	21 (25.3)	10 (30.3)	11 (22.0)
P53				0.281
Negative	31 (37.3)	10 (30.3)	21 (42.0)
Positive	52 (62.7)	23 (69.7)	29 (58.0)
HER2				0.283
Negative	63 (75.9)	23 (69.7)	40 (80.0)
Positive	20 (24.1)	10 (30.3)	10 (20.0)

P < 0.05 is considered statistically significant. HER2: Human epidermal growth factor receptor 2; P53: Protein 53; PAS: Periodic acid-Schiff.

Table 2 Univariate and multivariate Cox regression analysis based on clinicopathologic parameters for 83 signet ring cell carcinoma patients

	Univariate analysis			Multivariate analysis
	HR	95%CI	P value	HR	95%CI	P value
Age (yr)
≤ 60	1
> 60	1.461	0.624-3.418	0.382
Gender
Male	1
Female	0.865	0.384-1.949	0.727
Tumor diameter
≤ 3	1			1
> 3	4.428	1.750-11.199	0.002	2.761	1.086-7.020	0.033
Venous tumor emboli
No	1
Yes	3.634	1.351-9.775	0.011
Lymph node metastasis
No	1
Yes	3.006	1.120-8.066	0.029
Tumor stage
Early	1			1
Advanced	42.232	1.201-1485.160	0.039	159858	0.000-2.26E+153	0.945
Ki67
≤ 50%	1
> 50%	2.254	0.984-5.165	0.055
P53
Negative	1
Positive	2.532	0.995-6.443	0.051
HER2
Negative	1
Positive	1.232	0.487-3.114	0.659
PAS
> 50%	1			1
≤ 50%	5.284	2.164-12.905	0	3.809	0.108-0.640	0.003
AB
≤ 50%	1
> 50%	0.985	0.970-3.114	1.002
A/P
≤ 50%	1
> 0.5	1.402	1.028-1.910	0.033

P < 0.05 is considered statistically significant. AB: Alcian blue; A/P: Alcian blue to periodic acid Schiff ratio; CI: Confidence interval; HR: Hazard ratio; PAS: Periodic acid-Schiff.