ENTPD1-AS1–miR-144-3p-mediated high expression of COL5A2 correlates with poor prognosis and macrophage infiltration in gastric cancer

Table 1 Univariate analysis and multivariate analysis of correlation of COL5A2 expression among gastric cancer patients

Parameter	Univariate analysis		P value	Multivariate analysis		P value
	HR	95%CI		HR	95%CI
Age	1.022	1.004-1.039	0.014a	1.030	1.011-1.049	0.002b
Gender	1.372	0.941-2.001	0.100	1.289	0.873-1.903	0.202
Grade	1.364	0.963-1.930	0.080	1.397	0.974-2.005	0.069
Stae	1.576	1.268-1.959	< 0.001c	1.446	0.956-2.187	0.081
T	1.276	1.022-1.593	0.032a	0.954	0.702-1.295	0.761
M	1.809	0.973-3.364	0.061	1.475	0.677-3.214	0.328
N	1.343	1.147-1.573	< 0.001c	1.119	0.891-1.405	0.334
COL5A2	1.278	1.096-1.489	0.002b	2.321	1.215-4.434	0.011a

^aP < 0.05.

^bP < 0.01.

^cP < 0.001.

Table 2 Correlation analysis between long noncoding RNA and COL5A2 or long noncoding RNA and miR-144-3p in gastric cancer

lncRNA	miRNA/mRNA	R value	P value
ENTPD1-AS1	miR-144-3p	-0.324	1.6E-10c
ZNF460-AS1	miR-144-3p	-0.243	2.1E-06c
NORAD	miR-144-3p	-0.215	3.0E-05c
ENTPD1-AS1	COL5A2	0.109	3.6E-02a
ZNF460-AS1	COL5A2	-0.042	4.2E-01
NORAD	COL5A2	0.140	6.7E-03b

^aP < 0.05.

^bP < 0.01.

^cP < 0.001. lncRNA: Long noncoding RNA.

Table 3 Correlation analysis between COL5A2 and biomarkers of immune cells in gastric cancer

Immune cell	Biomarker	R value	P value
B cell	CD19	-0.053	3.1 × 10-1
	CD79A	0.008	8.8 × 10-1
CD8+ T cell	CD8A	0.078	1.3 × 10-1
	CD8B	-0.066	2.0 × 10-1
CD4+ T cell	CD4	0.312	8.4 × 10-10,c
M1 macrophage	NOS2	0.146	4.6 × 10-3,b
	IRF5	0.154	2.7 × 10-3,b
	PTGS2	0.373	1.1 × 10-13,c
M2 macrophage	CD163	0.466	< 2.2 × 10-16,c
	VSIG4	0.454	< 2.2 × 10-16,c
	MS4A4A	0.400	< 2.2 × 10-16,c
Neutrophil	CEACAM8	0.018	7.3 × 10-1
	ITGAM	0.443	< 2.2 × 10-16,c
	CCR7	0.072	1.7 × 10-1
Dendritic cell	HLA-DPB1	0.100	5.4 × 10-2
	HLA-DQB1	0.103	4.7 × 10-2,a
	HLA-DRA	0.118	2.3 × 10-2
	HLA-DPA1	0.096	6.2 × 10-2
	CD1C	0.064	2.2 × 10-1
	NRP1	0.568	< 2.2 × 10-16,c
	ITGAX	0.470	< 2.2 × 10-16,c

^aP < 0.05.

^bP < 0.01.

^cP < 0.001.