Basic Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Jul 15, 2023; 15(7): 1182-1199
Published online Jul 15, 2023. doi: 10.4251/wjgo.v15.i7.1182
ENTPD1-AS1–miR-144-3p-mediated high expression of COL5A2 correlates with poor prognosis and macrophage infiltration in gastric cancer
Han-Mei Yuan, Xiao-Feng Pu, Hui Wu, Chao Wu
Han-Mei Yuan, Hui Wu, Chao Wu, Department of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen 518033, Guangdong Province, China
Xiao-Feng Pu, Department of Clinical Laboratory, The General Hospital of Western Theater Command, Chengdu 610000, Sichuan Province, China
Author contributions: Yuan HM designed the experiments and wrote the manuscript; Pu XF and Wu H collected clinical specimens and completed the related experiments; Wu C reviewed and edited the manuscript; All authors contributed to the article and approved the submitted version.
Supported by National Natural Science Foundation of China, No. 81971489; and Natural Science Foundation of Guangdong Province, No. 2022A1515011122.
Institutional review board statement: The study was reviewed and approved by the Medical Research Ethics Committee of the Eighth Affiliated Hospital of Sun Yat-sen University, No. ZB-KYIRB-AF/SC-08/01.0.
Informed consent statement: All study participants or their legal guardian provided informed written consent about personal and medical data collection prior to study enrolment.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The original contributions presented in the study are included in the article/Supplementary material, further inquiries can be directed to the corresponding author.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Chao Wu, PhD, Professor, Department of Laboratory Medicine, The Eighth Affiliated Hospital, Sun Yat-sen University, No. 3025 Shennan Zhong Lu, Shenzhen 518033, Guangdong Province, China. chaowu261@163.com
Received: December 7, 2022
Peer-review started: December 7, 2022
First decision: January 18, 2023
Revised: March 30, 2023
Accepted: April 30, 2023
Article in press: April 30, 2023
Published online: July 15, 2023
Processing time: 217 Days and 1.5 Hours
Core Tip

Core Tip: Gastric cancer (GC) is a malignant tumor with high fatality rate. Competing endogenous RNA network and infiltration of immune cells play an important role in the development of GC. In this study, we verified that high expression of COL5A2 was closely related to poor prognosis and was an independent risk factor for GC. We predicted and validated that long noncoding RNA ENTPD1-AS1 regulated the expression of COL5A2 through sponging miR-144-3p. Additionally, we confirmed that upregulation of COL5A2 expression strongly correlated with immune infiltration of macrophages. ENTPD1-AS1-miR-144-3p-COL5A2 might be a new therapeutic target for GC.