Characterization of E-cadherin expression in normal mucosa, dysplasia and adenocarcinoma of gastric cardia and its influence on prognosis

Table 1 Clinical characteristics of patients with gastric cardia adenocarcinoma

Variables	Cases, n (%)
Gender
Female	954	20.9
Male	3607	79.1
Age at diagnosis (yr)
< 60	1717	37.6
≥ 60	2844	62.4
Family history
Negative	3366	73.8
Positive	1195	26.2
Cigarette smoking
No	2166	47.5
Yes	2395	52.5
Alcohol consumption
No	3206	70.3
Yes	1355	29.7
Differentiation
Well	133	2.9
Moderate	2039	44.7
Poor	2389	52.4
T status
T1	71	1.6
T2	308	6.8
T3	3044	66.7
T4	1138	25.0
Lymph node metastasis
Negative	1637	35.9
Positive	2924	64.1
Staging
Early stage	191	4.2
Advanced stage	4370	95.8

Table 2 The difference of E-cadherin protein expression in normal tissue, dysplasia and gastric cardia adenocarcinoma

Lesion type	Total	E-cadherin protein expression		χ2	P value
	n	Positive, n (%)	Negative, n (%)
Normal	208	208 (100.0)	0 (0)	47.439	< 0.001
DYS	156	145 (93.0)	11 (7.0)
GCA	4561	3836 (84.1)	725 (15.9)

DYS: Dysplasia; GCA: Gastric cardia adenocarcinoma.

Table 3 Association of E-cadherin expression with clinicopathological features in patients with gastric cardia adenocarcinoma

Variables	Total	E-cadherin protein expression		χ2	P value
	n	Positive, n (%)	Negative, n (%)
Gender				4.645	0.031
Female	954	824 (86.4)	130 (13.6)
Male	3607	3012 (83.5)	595 (16.5)
Age at diagnosis (yr)				0.709	> 0.05
< 60	1717	1434 (83.5)	283 (16.5)
≥ 60	2844	2402 (84.5)	442 (15.5)
Family history				1.018	> 0.05
Negative	3366	2820 (83.8)	546 (16.2)
Positive	1195	1016 (85.0)	179 (15.0)
Cigarette smoking				1.408	> 0.05
No	2166	1841 (85.0)	325 (15.0)
Yes	2395	2005 (83.7)	390 (16.3)
Alcohol consumption				0.011	> 0.05
No	3206	2706 (84.4)	500 (15.6)
Yes	1355	1142 (84.3)	213 (15.7)
Differentiation				14.259	0.001
Well	133	123 (92.5)	10 (7.5)
Moderate	2039	1742 (85.4)	297 (14.6)
Poor	2389	1971 (82.5)	418 (17.5)
T status				22.490	< 0.001
pT1	71	63 (88.7)	8 (11.3)
pT2	308	278 (90.3)	30 (9.7)
pT3	3044	2580 (84.8)	464 (15.2)
pT4	1138	915 (80.4)	223 (19.6)
Lymph node metastasis				0.481	> 0.05
Negative	1637	1385 (84.6)	252 (15.4)
Positive	2924	2451 (83.8)	473 (16.2)
Staging				10.941	0.001
Early stage	191	177 (92.7)	14 (7.3)
Advanced stage	4370	3659 (83.7)	711 (16.3)

Table 4 Univariate and multivariate analyses of survival of patients with gastric cardia adenocarcinoma

Variables	Univariate analysis			Multivariate analysis
	HR	95%CI	P value	HR	95%CI	P value
Gender			> 0.05
Female	1
Male	1.063	0.979-1.153
Age at diagnosis (yr)			< 0.001			< 0.001
< 60	1			1
≥ 60	1.335	1.246-1.431		1.352	1.262-1.449
Family history			> 0.05
Negative	1
Positive	1.060	0.983-1.143
Cigarette smoking			> 0.05
No	1
Yes	1.004	0.929-1.084
Alcohol consumption			> 0.05
No	1
Yes	0.947	0.869-1.031
Differentiation			< 0.001			< 0.001
Well	1			1
Moderate	1.316	1.067-1.623		1.234	1.000-1.522
Poor	1.791	1.454-2.206		1.480	1.199-1.827
T status			< 0.001			< 0.001
pT1	1			1
pT2	1.916	1.289-2.849		1.604	1.078-2.387
pT3	2.829	1.949-4.107		2.074	1.426-3.018
pT4	3.390	2.328-4.936		2.272	1.555-3.320
Lymph node metastasis			< 0.001			< 0.001
Negative	1			1
Positive	1.952	1.815-2.099		1.805	1.676-1.944
E-cadherin			0.003			0.026
Positive	1			1
Negative	1.144	1.048-1.248		1.104	1.012-1.206

CI: Confidence interval; GCA: Gastric cardia adenocarcinoma; HR: Hazard ratio.