Direct interaction between Rab5a and Rab4a enhanced epidermal growth factor-stimulated proliferation of gastric cancer cells

Table 1 Expression levels of Rab5a and Rab4a in gastric cancer tissues with different clinicopathological characteristics

Classification		Clinical stages		Histological classification		Metastasis
		Advanced	Early	I + II	III + IV	Yes	No
Number of cases		22	20	27	15	22	20
Gender	Male	18	12	18	12	16	14
	Female	4	8	9	3	6	6
Age		60 ± 11	62 ± 17	59 ± 12	62 ± 11	63 ± 10	57 ± 13
Rab5	-	2	8	9	0	0	6
	+	6	10	11	4	12	10
	++	10	2	7	8	10	4
	+++	4	0	0	3	2	0
	Statistics (χ2)	13.87		12.963		10.476
	P value	< 0.01		< 0.01		0.015
Rab4	-	0	6	7	0	0	8
	+	6	12	12	6	4	8
	++	12	2	8	6	12	4
	+++	4	0	0	3	6	0
	Statistics (χ2)	19.091		9.644		19.282
	P value	< 0.01		0.022		< 0.01

Statistical analysis of expression levels of Rab5a, Rab4a between difference stages of clinical stages (early stage and advanced stage), between histological grades (I + II and III + IV), and between metastasis condition (yes and no) by Kruskal-Wallis H Test.

Table 2 Expression levels of Rab5a, Rab4a, leucine zipper motif 1, and histone deacetylase 1 in normal gastric mucosa, gastric tissues adjacent to the tumors, early-stage gastric cancer tumor tissues, and advanced-stage gastric cancer tissues as determined by immunochemical analysis

	HDAC1				APPL1				Rab4a				Rab5a
	-	+	++	+++	-	+	++	+++	-	+	++	+++	-	+	++	+++
Normal gastric tissue	20	0	0	0	12	8	0	0	18	2	0	0	16	4	0	0
Paracancerous tissue	24	10	0	0	24	10	0	0	20	14	0	0	22	12	0	0
Early-stage gastric cancer	4	8	8	0	5	10	5	0	6	12	2	0	8	10	2	0
Advanced-stage gastric cancer	0	8	10	4	2	6	10	4	0	6	12	4	2	6	10	4
Statistics (χ2)	59.162				43.456				58.338				45.719
P value	< 0.001				< 0.001				< 0.001				< 0.001

Statistical analysis of expression levels of Rab5a, Rab4a, leucine zipper motif 1, histone deacetylase 1 between four groups of normal, paracancerous, early-stage gastric cancer, and advanced-stage gastric cancer tissues by Kruskal-Wallis H Test. HDAC1: Histone deacetylase 1; APPL1: Leucine zipper motif 1.