shRNA-interfering LSD1 inhibits proliferation and invasion of gastric cancer cells via VEGF-C/PI3K/AKT signaling pathway

Table 1 Relationship between Lysine Specific Demethylase 1 expression and clinicopathological parameters in 50 cases of gastric cancer

Pathological parameters	n	Low LSD1 expression	High LSD1 expression	P value
Gender				0.779
Male	29	12	17
Female	21	12	9
Age (yr)				0.528
≥ 60	23	13	10
≤ 60	27	8	19
Tumor diameter (cm)				0.143
> 5	26	19	7
≤ 5	24	11	13
Local lymph node metastasis				0.006
Yes	18	9	9
No	32	10	22
Infiltration depth				0.285
T1	9	3	6
T2	8	5	3
T3	23	5	18
T4	10	3	7
Differentiation				0.026
high	26	6	20
medium	14	2	12
low	10	3	7
Duke stage				0.017
A	10	1	9
B	13	3	10
C	9	0	9
D	17	4	13

LSD1: Lysine Specific Demethylase 1.

Table 2 Gray values of two groups of MKN-45 cells (x ± SD, n = 10)

	Control group	LSD1-shRNA group
mRNA expression	0.33 ± 0.05	0.25 ± 0.015 a
Protein expression	0.963 ± 0.037	0.63 ± 0.02a

^aP < 0.05 vs the control group. LSD1: Lysine Specific Demethylase 1.

Table 3 OD values of two groups of MKN-45 cells (x ± SD, n = 3)

Time (h)	Control group	LSD1-shRNA group
0	0.362 ± 0.005	0.315 ± 0.006
24	0.534 ± 0.011	0.455 ± 0.013
48	0.792 ± 0.009	0.527 ± 0.008a
72	0.891 ± 0.005	0.599 ± 0.006a
96	0.914 ± 0.013	0.589 ± 0.012 a

^aP < 0.05 vs control group. LSD1: Lysine Specific Demethylase 1.