Copyright
©The Author(s) 2019.
World J Gastrointest Oncol. Nov 15, 2019; 11(11): 933-945
Published online Nov 15, 2019. doi: 10.4251/wjgo.v11.i11.933
Published online Nov 15, 2019. doi: 10.4251/wjgo.v11.i11.933
Table 1 Main pitfalls towards the optimization and standardization of the use of endoscopic ultrasound-obtained material for molecular investigations
| Endoscopic ultrasound obtained material for molecular investigations | Main pitfalls |
| DNA and RNA extraction and use for molecular investigations | Needle choice |
| Sample storage | |
| Cellularity and contamination | |
| RNA degradation | |
| Intratumoral heterogeneity | |
| Total amount of tumoral cells | |
| Lesional-to-non-lesional cell ratio | |
| Molecular modifications during disease course | |
| Generation of patient-derived xenografts | Expensive |
| Time- and resource-consuming | |
| Low engraftment rates | |
| Generation of organoids | Lack of standardization |
| Lack of immune cells, blood vessels, and stromal components |
- Citation: Archibugi L, Testoni SGG, Redegalli M, Petrone MC, Reni M, Falconi M, Doglioni C, Capurso G, Arcidiacono PG. New era for pancreatic endoscopic ultrasound: From imaging to molecular pathology of pancreatic cancer. World J Gastrointest Oncol 2019; 11(11): 933-945
- URL: https://www.wjgnet.com/1948-5204/full/v11/i11/933.htm
- DOI: https://dx.doi.org/10.4251/wjgo.v11.i11.933