Case Control Study Open Access
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Aug 15, 2023; 15(8): 1424-1435
Published online Aug 15, 2023. doi: 10.4251/wjgo.v15.i8.1424
Fecal microbial biomarkers combined with multi-target stool DNA test improve diagnostic accuracy for colorectal cancer
Jin-Qing Fan, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Ningbo University, Ningbo 315000, Zhejiang Province, China
Wang-Fang Zhao, Qi-Wen Lu, Guo-Liang Ye, Department of Gastroenterology, The First Affiliated Hospital of Ningbo University, Ningbo 315000, Zhejiang Province, China
Fu-Rong Zha, Department of Bioinformation Analysis, Shanghai BIOZERON Biotechnology Co., Shanghai 201800, China
Le-Bin Lv, Han-Lu Gao, Department of Preventive Medicine, The First Affiliated Hospital of Ningbo University, Ningbo 315000, Zhejiang Province, China
ORCID number: Jin-Qing Fan (0000-0001-6985-3261); Guo-Liang Ye (0000-0003-0600-9981); Han-Lu Gao (0000-0002-1066-0155).
Author contributions: Fan JQ collected the clinical data and wrote the original manuscript; Zhao WF, Lu QW, and Ye GL participated in the collection of human material; Lv LB performed data collection and collation; Zha FR performed bioinformatics analysis; Gao HL conceived the research and edited the manuscript.
Supported by the Medical and Health Research Project of Zhejiang Province, No. 2021KY1048 and 2022KY1142; Ningbo Health Young Technical Backbone Talents Training Program, No. 2020SWSQNGG-02; and the Key Science and Technology Project of Ningbo City, No. 2021Z133.
Institutional review board statement: The study was approved by the Human Research and Ethics Committee of the Affiliated Hospital of Medical School of Ningbo University (approval number: KY20211104).
Informed consent statement: All the participants provided written informed consent.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
STROBE statement: The authors have read the STROBE statement, and the manuscript was prepared and revised according to the STROBE statement.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Han-Lu Gao, PhD, Doctor, Department of Preventive Medicine, The First Affiliated Hospital of Ningbo University, No. 247 Renmin Road, Ningbo 315000, Zhejiang Province, China. 306646058@qq.com
Received: March 24, 2023
Peer-review started: March 24, 2023
First decision: May 19, 2023
Revised: May 20, 2023
Accepted: June 19, 2023
Article in press: June 19, 2023
Published online: August 15, 2023
Processing time: 139 Days and 5 Hours

Abstract
BACKGROUND

Colorectal cancer (CRC) is a major global health burden. The current diagnostic tests have shortcomings of being invasive and low accuracy.

AIM

To explore the combination of intestinal microbiome composition and multi-target stool DNA (MT-sDNA) test in the diagnosis of CRC.

METHODS

We assessed the performance of the MT-sDNA test based on a hospital clinical trial. The intestinal microbiota was tested using 16S rRNA gene sequencing. This case-control study enrolled 54 CRC patients and 51 healthy controls. We identified biomarkers of bacterial structure, analyzed the relationship between different tumor markers and the relative abundance of related flora components, and distinguished CRC patients from healthy subjects by the linear discriminant analysis effect size, redundancy analysis, and random forest analysis.

RESULTS

MT-sDNA was associated with Bacteroides. MT-sDNA and carcinoembryonic antigen (CEA) were positively correlated with the existence of Parabacteroides, and alpha-fetoprotein (AFP) was positively associated with Faecalibacterium and Megamonas. In the random forest model, the existence of Streptococcus, Escherichia, Chitinophaga, Parasutterella, Lachnospira, and Romboutsia can distinguish CRC from health controls. The diagnostic accuracy of MT-sDNA combined with the six genera and CEA in the diagnosis of CRC was 97.1%, with a sensitivity and specificity of 98.1% and 92.3%, respectively.

CONCLUSION

There is a positive correlation of MT-sDNA, CEA, and AFP with intestinal microbiome. Eight biomarkers including six genera of gut microbiota, MT-sDNA, and CEA showed a prominent sensitivity and specificity for CRC prediction, which could be used as a non-invasive method for improving the diagnostic accuracy for this malignancy.

Key Words: Gut microbiome; Colorectal cancer; Diagnostic model; Multi-target stool DNA test; Tumor biomarker

Core Tip: There is a positive correlation of multi-target stool DNA (MT-sDNA), carcinoembryonic antigen (CEA), and alpha-fetoprotein with intestinal microbiome. Eight biomarkers including six genera of gut microbiota, MT-sDNA, and CEA showed a prominent sensitivity (98.1%) and specificity (92.3%) for colorectal cancer prediction, which could be used as a non-invasive method for improving the diagnostic accuracy for this malignancy.



INTRODUCTION

Colorectal cancer (CRC) is the third most common cancer and the fifth-leading cause of cancer-related deaths in China[1]. Although colonoscopy is considered the gold standard in the diagnosis of CRC, it has shortcomings of being invasive and expensive[2]. Thus, non-invasive and effective diagnostic methods are needed urgently. Tumor markers such as carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA199), and alpha-fetoprotein (AFP) are not specific and sensitive in detecting CRC, which may lead to delayed treatment and reduced survival rates[3]. Fortunately, increasing evidence suggests that gut bacteria play an important role in the development of CRC[4,5]. Fecal bacteria can be used as non-invasive biomarkers for CRC diagnosis[6]. Combined tests for different intestinal bacteria in CRC had a sensitivity and specificity ranging from 54.0%-84.6%, and 63.1%-90.0%, respectively[7,8]. Due to the complexity of the gut microbiome, the diagnosis accuracy of the current tests for CRC is still inadequate. Therefore, a combination screening method with high specificity and sensitivity should be developed for early detection of CRC. Recently, the multi-target stool DNA (MT-sDNA) test has been recommended for CRC screening and applied in the commercial market[9]. Due to the difference in gut microbiota between CRC patients and healthy controls (HC), combined MT-sDNA test and tumor biomarkers are required for improving diagnostic accuracy[10]. This study focused on the combination of intestinal microbiome composition and MT-sDNA test in an attempt to develop new detection methods for the diagnosis of CRC.

MATERIALS AND METHODS
Participant enrollment

A total of 105 participants with an age range from 40 to 74 years who visited the anorectal department and health examination center of the Affiliated Hospital of Medical School of Ningbo University from January 2021 to November 2022 were recruited.

The inclusion criteria for patients with CRC were: (1) Newly diagnosed sporadic CRC patients with an expected survival > 3 mo; and (2) The patients did not undergo colorectal surgery or chemoradiotherapy. The inclusion criteria for HC were: The subjects underwent colonoscopy and the results were normal.

The exclusion criteria were: (1) Subjects with chronic diarrhea; (2) Subjects with tumor metastasis or recurrence; (3) Subjects with a family history of CRC; (4) Subjects with inflammatory bowel disease, obesity, diabetes, or other metabolic diseases; (5) Subjects with preoperative radiotherapy and chemotherapy; (6) Subjects who had taken laxatives, antibiotics, microecological preparations, and other drugs in the past 1 mo; (7) Subjects with cirrhosis; and (8) Subjects with colorectal polyps.

All participants provided informed consent and the study was approved by the Human Research and Ethics Committee of the Affiliated Hospital of Medical School of Ningbo University (approval number: KY20211104). This study conforms to the principles of the Declaration of Helsinki in 2013.

MT-sDNA tests

Fecal samples (4-5 g) were collected prior to bowel preparation for colonoscopy and before surgical removal of intestinal tumor tissue from CRC patients. All experimental procedures related to the MT-sDNA tests [fecal immunochemical test (FIT), NDRG4 and BMP3 methylation, KRAS mutation] were carried out using the commercial kit ColoClear® (New Horizon Health Technology, Hangzhou, China). The details regarding probes and primers, as well as the risk prediction algorithm, were the same as those described in a previous report[11]. In this MT-sDNA risk prediction model, a risk score was provided as a single output. If the risk score value was ≥ 165, the test was considered “positive”. If the risk score was < 165, the test was regarded as “negative”[12].

Detection of intestinal microbiota

Fresh fecal samples (≥ 1 g) from all participants were collected prior to colonoscopy and frozen at -80 °C immediately. Total microbial genomic DNA was extracted from the fecal samples using E.Z.N.A®fecal DNA Kit (Omega, United States) according to the manufacturer’s recommendations. The sequences of the V1-V9 variable regions of the bacterial 16S rRNA gene were amplified using primers 27F (5’-AGRGTTYGATYMTGGCTCAG-3’) and 1492R (5’-RGYTACCTTGTTACGACTT-3’)[13]. The polymerase chain reaction (PCR) conditions to amplify the prokaryotic 16S fragments were consistent with those described in the previous literature[14]. The PCR amplication products were confirmed by 2% agarose gel electrophoresis and purified with AxyPrep DNA Gel Extraction Kit (Axygen Biosciences, United States). We conducted 16S rDNA sequencing on the PacBio Sequel IIe platform (Biozeron Biotechnology, Shanghai, China).

Clinical procedures

Three serum biomarkers, i.e., CA199, CEA, and AFP, were determined by the Laboratory of the Affiliated Hospital of Ningbo University School of Medicine. Histological diagnosis and colonoscopy findings were used as the reference for determining the accuracy of the combination test for validating screening performance. All pathological diagnoses were in accordance with the diagnostic criteria of the 2010 World Health Organization Classification of Gastrointestinal Neoplasms.

Sequencing data and statistical analysis

The complexity of sample species diversity was analyzed by alpha diversity, and the richness and diversity of community were described by Shannon index and Simpson index. Beta diversity analysis was used to compare the samples and was evaluated by principal component analysis (PCA). Alpha diversity and beta diversity analyses were conducted using USEARCH software (version 1.1). Linear discriminant analysis (LDA) effect size (LEfSe) analysis was conducted to identify biomarkers of bacterial structure in the case-control groups[15]. Kruskal-Wallis and rank tests were used to analyze the changes and differences among the classes, and LDA was used to determine the size effects of each significant abundance group[16]. The relationship between intestinal flora, MT-sDNA, and tumor markers was analyzed by redundancy analysis (RDA). We built the random forest model to distinguish patients with CRC from HC. The analysis of disease diagnosis included the calculation of disease diagnosis index and the evaluation of diagnosis effect. Diagnostic index calculation was based on the differential genus identified in the random forest analysis to obtain the disease diagnostic index of the sample. Receiver operating characteristic (ROC) curve was used to evaluate the diagnostic effect of disease diagnostic index. The sensitivity and specificity were analyzed by ROC curve analysis with the area under the ROC curve (AUC) and 95% confidence interval calculated for the tumor markers, MT-sDNA, and intestinal flora. The analysis process was completed using vegan package, random forest package, and plotROC of R software (version 4.2, United States). t-test and chi-square test were adopted to compare the differences between different groups. P < 0.05 was considered statistically significant. Statistical analyses were performed using SPSS software (version 23.0, United States).

RESULTS
Basic demographic and clinical characteristics

One patient was excluded from the study because of missing the sample storage standard, and 105 subjects were included eventually (Figure 1). The clinical features of the 105 participants are shown in Table 1. A total of 54 CRC patients and 51 HC were enrolled in this research, with an average age and standard deviation of 64.89 ± 9.72 and 53.94 ± 10.33, separately. The colon was the most common site (66.67%) in CRC patients. Moderate/low differentiation, ulcerative type, and Dukes stage A accounted for 92.59%, 61.11%, and 79.63% of CRC cases, respectively. There were statistically differences in terms of age, education level, and body mass index between the two groups (P < 0.05).

Figure 1
Figure 1 Flow chart of participant selection. CRC: Colorectal cancer; MT-sDNA: Multi-target stool DNA; FMDI: Fecal microbiota diagnostic index; CEA: Carcinoembryonic antigen; AFP: Alpha-fetoprotein; CA199: Carbohydrate antigen 199; PCA: Principal component analysis; RDA: Redundancy analysis; LEfSe: Linear discriminant analysis effect size.
Table 1 Basic demographic and clinical characteristics of cases and controls.
Variable
Colorectal cancer patients
Health controls
P value
Gender0.30
Male34 (62.96)27 (52.94)
Female20 (37.04)24 (47.06)
Age0.00
mean ± SD64.89 ± 9.7253.94 ± 10.33
< 60 yr18 (33.33)35 (68.63)
≥ 60 yr36 (66.67)16 (31.37)
Education level0.00
Junior high school and below43 (79.63)10 (19.61)
Senior high school and above11 (20.37)41 (80.39)
BMI (kg/m2)0.00
≤ 18.54 (7.41)0 (0.00)
18.5-23.937 (68.52)24 (47.06)
≥ 2413 (24.07)27 (52.94)
Tumor location
Colon36 (66.67)--
Rectum18 (33.33)--
Pathogenic type--
Protrude type10 (18.52)--
Infiltrating type11 (20.37)--
Ulcerative type33 (61.11)--
Differentiation--
High4 (7.41)--
Medium/low50 (92.59)--
Histological type--
Adenocarcinoma54 (100.00)--
Other types0 (0.00)--
Dukes stage--
A43 (79.63)--
B7 (12.96)--
C4 (7.41)--
D0 (0.0)--
Comparison of tumor marker expression level between the two groups

As shown in Table 2, the levels of tumor biomarkers CEA and CA199 and the DNA score were elevated in CRC patients compared with HC (P < 0.05). Fecal microbiota diagnostic index (FMDI) was -215.76 ± 539.49 and 178.47 ± 249.43 in CRC patients and HC (P < 0.05), respectively.

Table 2 Evaluation of tumour marker expression, DNA score and fecal microbiota diagnostic index between cases and controls.
Variable
Colorectal cancer patients
Health controls
P value
AFP
mean ± SD5.70 ± 17.103.61 ± 1.590.27
≤ 7 μg/L49 (90.74)49 (96.08)
> 7.1 μg/L5 (9.26)2 (3.92)
CEA
mean ± SD40.67 ± 154.342.22 ± 1.470.00
≤ 5 μg/L38 (70.37)48 (94.12)
> 5.1 μg/L16 (29.63)3 (5.88)
CA1990.02
mean ± SD59.65 ± 269.5910.01 ± 9.26
≤ 25 μg/mL44 (81.48)49 (96.08)
> 25.1 μg/mL10 (18.52)2 (3.92)
DNA score
mean ± SD787.87 ± 283.7194.39 ± 41.620.00
< 1655 (9.26)50 (98.04)
≥ 16549 (90.74)1 (1.96)
Distribution of intestinal flora in the two groups

The number of OTUs obtained in this research was 453, among which 93 unique OTUs belonged to the CRC group and 76 unique OTUs existed in the HC group. There were 284 OTUs shared between the CRC and HC groups, and details are shown in a Venn diagram (Supplementary Figure 1A).

There was no statistical difference in alpha diversity between the CRC and control groups. The Shannon index (P = 0.79) and Simpson index (P = 0.50) results are shown in Supplementary Figures 1B and C. In addition, PCA plot showed no difference in beta diversity of gut microbiota (Supplementary Figure 1D).

LEfSe results in the two groups

In order to find species with significant differences in abundance in the two groups, the cladistic diagram of LEfSe analysis was used, as shown in Figure 2. LEfSe analysis identified 23 characteristic bacterial groups (Figure 2A). An LDA score > 4 indicated a significant difference in structure between the two groups (Figure 2B). Fusobacterium, Bacteroides, Enterobacterales, Gammaproteobacteria, Proteobacteria, and Escherichia were more abundant in CRC patients than in HC. The abundance of Prevotellaceae, Lachnospiraceae, Oscillospiraceae, Eubacteriales, and Clostridia was higher in the HC group.

Figure 2
Figure 2 Differences in fecal microbiota between colorectal cancer patients and healthy controls. A: The clustering tree with red areas and green areas represents different groups. The red nodes in the branches represent microbial groups that play an important role in the red group, the green nodes represent microbial groups that play an important role in the green group, and the yellow nodes represent microbial groups that do not play an important role in either group. The genus names are shown in the legend on the right; B: Linear discriminant analysis (LDA) score histogram to identify diverse bacterial genus (LDA score ≥ 4, P < 0.05). CRC: Colorectal cancer.
Correlation analysis between bacterial community morphology and tumor biomarkers

In order to analyze the relationship between different tumor markers and the relative abundance of related flora components and to observe the correlation between different flora and tumor biomarkers, RDA was performed. The results of RDA analysis showed positive correlations between MT-sDNA and Bacteroides. Parabacteroides was positively correlated with MT-sDNA and CEA, and Faecalibacterium and Megamonas was positively associated with AFP (Figure 3).

Figure 3
Figure 3 Redundancy analysis in bacterial community characteristics (genus level) and tumor markers. Dots in the figure represent sample names; arrows indicate tumor biomarkers; inverted triangle represents species. CRC: Colorectal cancer; CEA: Carcinoembryonic antigen; AFP: Alpha-fetoprotein; CA199: Carbohydrate antigen 199; PCA: Principal component analysis; RDA: Redundancy analysis.
Diagnostic value of intestinal flora, MT-sDNA, and tumor markers in CRC

The random forest model results showed that the combination of the six genera, namely, Streptococcus, Escherichia, Chitinophaga, Parasutterella, Lachnospira, and Romboutsia (Figure 4), can distinguish CRC patients from HC. We assessed the clinical diagnostic value of intestinal microbiome, tumor markers, and MT-sDNA in CRC patients. We found that the sensitivity, specificity, and AUC of intestinal microbiome were lower than those of the combined detection of MT-sDNA and intestinal microbiome in CRC (AUC = 84.8% and 95.4%, respectively). The specificity and sensitivity of intestinal microbiome combined with MT-sDNA and CEA in the diagnosis of CRC were 92.3% and 98.1%, and the diagnostic accuracy was 97.1%, which was higher than that of either of the tumor biomarkers or MT-sDNA test alone (94.3% and 83.0%, respectively) (Figure 5).

Figure 4
Figure 4 The biomarker identification results by random forest model. The lattice plots show the identified biomarkers and their importance, with retention importance higher than 1.5.
Figure 5
Figure 5 Receiver operating characteristic curves of different detection methods to assess colorectal cancer. CEA: Carcinoembryonic antigen; AFP: Alpha-fetoprotein; CA199: Carbohydrate antigen 199; PCA: Principal component analysis; AUC: Area under the receiver operating characteristic curve.
DISCUSSION

Most of CRC cases are sporadic and follow a pattern of adenomatous to cancerous progression[17]. The development of CRC is caused by the interaction of genetic and environmental factors, and changes in intestinal microbiome are closely related to CRC.

Previous studies have confirmed changes in the bacterial composition of CRC, but no effective diagnostic model has been developed in the aspect of MT-sDNA, gut microbiota, and tumor biomarkers interactions. We performed 16S rDNA sequencing to investigate the differences in gut microbiota between patients with CRC and HC. In our study, Fusobacterium, Bacteroides, Gammaproteobacteria, Proteobacteria, Escherichia, and Enterobacterales were more abundant in CRC patients than in HC. The intestinal flora of CRC was mainly Bacteroides and Fusobacterium as found in previous studies[18-20]. Fusobacterium and Bacteroides are associated with metastasis and poor survival outcomes in CRC patients, which makes them emerging candidate biomarkers[21,22]. We speculate that the gathering of Fusobacterium may result in colonization of the colon mucosa and further promote the occurrence of CRC, suggesting that Fusobacterium may be a “driving factor” for the occurrence of CRC[23]. Bacteroides promote tumorigenesis by inducing cell proliferation and promoting tumor inflammation through toxins[24]. Additionally, Proteobacteria, Escherichia, Gammaproteobacteria, and Enterobacterales were also affirmed as the CRC-associated bacteria in previous studies[25]. Proteobacteria are colonized in the gut, causing persistent intestinal inflammation[26]. Escherichia may influence the development and progression of CRC through inflammatory pathways and virulence factors[27]. Gammaproteobacteria could break leupeptin protease inhibitors and was related to colonization phenotypes[28]. Enterobacterales induced the cell death, and contained apoptosis-inducing substances[29]. Therefore, the gut microbiome has a direct pathogenic role in cell proliferation, cell death, and inflammation in CRC, suggesting that regulating the gut microbiome can be a powerful tool for CRC prevention and treatment.

Tumor markers can be used to predict the diagnosis of CRC[30]. Previous research showed that CEA and CA199 yielded an AUC of 0.74 and 0.67 to discriminate HC from CRC patients, which was similar with our results[31]. The ability of tumor markers to distinguish CRC is limited, and new biomarkers need to be found for the diagnosis of CRC. To achieve a comprehensive analysis, we first investigated the association between microbial composition and tumor markers in CRC patients. We found positive correlations between MT-sDNA and Bacteroides. Parabacteroides was positively correlated with MT-sDNA and CEA, and Faecalibacterium and Megamonas were positively associated with AFP. This suggests that gut microbiota composition affects the MT-sDNA and tumor biomarkers in CRC patients. The results of this study provide new ideas for tumor markers and intestinal flora in the drug therapy of CRC. Second, the random forest model results showed that the combination of the six genera, namely, Streptococcus, Escherichia, Chitinophaga, Parasutterella, Lachnospira, and Romboutsia, can distinguish CRC from HC. The results suggested that these six genera could be used as the potential biomarkers for CRC diagnosis. Similar to other studies, our research confirmed that the sensitivity of MT-sDNA and fecal bacteria in terms of CRC diagnosis was relatively high[32,33], indicating that MT-sDNA and gut microbiota are suitable for the diagnosis of CRC[34,35]. Previous studies mostly focused on the diagnosis accuracy of MT-sDNA and intestinal microbiome detection alone or combined with FIT, which is lacking diagnostic accuracy. There was no studies focused on the combination of MT-sDNA test, intestinal microbiome, and tumor markers in the diagnosis of CRC. We found that, in the detection of CRC, the sensitivity and accuracy of MT-sDNA combined with CEA and fecal bacteria increased, which indicated that this combination has robust advantages in distinguishing CRC patients from HC.

Although our study suggests that intestinal flora combined with tumor markers can improve the diagnostic efficacy for CRC, our study have some shortcomings. First, the number of subjects included in our research was not large enough and no multicenter clinical validation was performed. A larger sample size is expected to lead to more discoveries in the future. Second, further experimental studies on mechanism investigations are needed to confirm our findings and investigate the effects of tumor markers, MT-sDNA, and intestinal flora on CRC patients. Third, environmental factors such as diet and lifestyle were not investigated in this study, and the influence of environmental factors on intestinal flora could not be analyzed. However, our findings in this study will optimize the diagnosis of CRC and provide new ideas to transform microbita-based strategies into precise diagnosis in the clinic.

CONCLUSION

Our study reveals that CRC-associated bacteria have specific differences. There are positive correlations of MT-sDNA, CEA, as well as AFP with intestinal microbiome. Fecal microbiological markers combined with MT-sDNA and CEA can enhance the sensitivity and specificity for the diagnosis of CRC, and are feasible in clinical application.

ARTICLE HIGHLIGHTS
Research background

Colorectal cancer (CRC) is a major global health burden, and its incidence and mortality have increased rapidly over the past decades and resulted in massive economic burdens in China.

Research motivation

This case-control study enrolled 54 CRC patients and 51 healthy controls.

Research objectives

This research aimed to explore the characteristics of intestinal flora and its correlation with multi-target stool DNA (MT-sDNA) and tumor markers in CRC patients, and evaluate the diagnostic performance of MT-sDNA and tumor biomarkers combined with microbiota in CRC.

Research methods

We evaluated the performance of the MT-sDNA test based on a hospital clinical trial. The intestinal microbiota was tested using 16S rRNA gene sequencing. We identified biomarkers of bacteria structure, analyzed the relationship between different tumor markers and the relative abundance of related flora components, and distinguished CRC patients from healthy subjects by the linear discriminant analysis effect size, redundancy analysis, and random forest analysis, respectively.

Research results

We found that MT-sDNA was closely associated with Bacteroides. MT-sDNA and carcinoembryonic antigen (CEA) were positively correlated with the existence of Parabacteroides, and alpha-fetoprotein was positively associated with Faecalibacterium and Megamonas. The random forest model results showed that the combination of the six genera, namely, Streptococcus, Escherichia, Chitinophaga, Parasutterella, Lachnospira, and Romboutsia, can distinguish CRC from health controls. The sensitivity and specificity of MT-sDNA combined with the six genera and CEA in the diagnosis of CRC were 98.1% and 92.3%, respectively, and the diagnostic accuracy was 97.1%.

Research conclusions

MT-sDNA and tumor markers were positively correlated with intestinal flora. Intestinal flora, MT-sDNA, and tumor markers showed significant sensitivity and specificity for CRC prediction, which could be used as a non-invasive method to improve the diagnostic accuracy.

Research perspectives

Our results will optimize the diagnosis of CRC and provide new ideas for translating microbit-based diagnostic strategies into precise diagnosis in the clinic.

Footnotes

Provenance and peer review: Unsolicited article; Externally peer reviewed.

Peer-review model: Single blind

Specialty type: Oncology

Country/Territory of origin: China

Peer-review report’s scientific quality classification

Grade A (Excellent): 0

Grade B (Very good): 0

Grade C (Good): C, C

Grade D (Fair): 0

Grade E (Poor): 0

P-Reviewer: Gazouli M, Greece; Jamali R, Iran S-Editor: Wang JJ L-Editor: Wang TQ P-Editor: Zhang XD

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