Case Control Study
Copyright ©The Author(s) 2023. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastrointest Oncol. Aug 15, 2023; 15(8): 1424-1435
Published online Aug 15, 2023. doi: 10.4251/wjgo.v15.i8.1424
Fecal microbial biomarkers combined with multi-target stool DNA test improve diagnostic accuracy for colorectal cancer
Jin-Qing Fan, Wang-Fang Zhao, Qi-Wen Lu, Fu-Rong Zha, Le-Bin Lv, Guo-Liang Ye, Han-Lu Gao
Jin-Qing Fan, Department of Traditional Chinese Medicine, The First Affiliated Hospital of Ningbo University, Ningbo 315000, Zhejiang Province, China
Wang-Fang Zhao, Qi-Wen Lu, Guo-Liang Ye, Department of Gastroenterology, The First Affiliated Hospital of Ningbo University, Ningbo 315000, Zhejiang Province, China
Fu-Rong Zha, Department of Bioinformation Analysis, Shanghai BIOZERON Biotechnology Co., Shanghai 201800, China
Le-Bin Lv, Han-Lu Gao, Department of Preventive Medicine, The First Affiliated Hospital of Ningbo University, Ningbo 315000, Zhejiang Province, China
Author contributions: Fan JQ collected the clinical data and wrote the original manuscript; Zhao WF, Lu QW, and Ye GL participated in the collection of human material; Lv LB performed data collection and collation; Zha FR performed bioinformatics analysis; Gao HL conceived the research and edited the manuscript.
Supported by the Medical and Health Research Project of Zhejiang Province, No. 2021KY1048 and 2022KY1142; Ningbo Health Young Technical Backbone Talents Training Program, No. 2020SWSQNGG-02; and the Key Science and Technology Project of Ningbo City, No. 2021Z133.
Institutional review board statement: The study was approved by the Human Research and Ethics Committee of the Affiliated Hospital of Medical School of Ningbo University (approval number: KY20211104).
Informed consent statement: All the participants provided written informed consent.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.
STROBE statement: The authors have read the STROBE statement, and the manuscript was prepared and revised according to the STROBE statement.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Han-Lu Gao, PhD, Doctor, Department of Preventive Medicine, The First Affiliated Hospital of Ningbo University, No. 247 Renmin Road, Ningbo 315000, Zhejiang Province, China. 306646058@qq.com
Received: March 24, 2023
Peer-review started: March 24, 2023
First decision: May 19, 2023
Revised: May 20, 2023
Accepted: June 19, 2023
Article in press: June 19, 2023
Published online: August 15, 2023
Processing time: 139 Days and 5 Hours
Core Tip

Core Tip: There is a positive correlation of multi-target stool DNA (MT-sDNA), carcinoembryonic antigen (CEA), and alpha-fetoprotein with intestinal microbiome. Eight biomarkers including six genera of gut microbiota, MT-sDNA, and CEA showed a prominent sensitivity (98.1%) and specificity (92.3%) for colorectal cancer prediction, which could be used as a non-invasive method for improving the diagnostic accuracy for this malignancy.