Characterization and strong risk association of TLR2 del -196 to -174 polymorphism and Helicobacter pylori and their influence on mRNA expression in gastric cancer

Figure 1 Relative gene expression levels of TLR2. A: Comparison between gastric cancer (GC) and chronic gastritis groups (CG), ^aP < 0.0001; and GC or CG groups with samples of normal mucosa (RQ = 1 represented for dashed line), ^bP < 0.0001 and P = 0.9387, respectively. B: Individual representation of RQ for each sample of GC group; C: Individual representation of relative quantification (RQ) for each sample of CG group. Significant difference, ^{a, b}P < 0.05. RQ represented by median with interquartile range. Mann-Whitney and Wilcoxon Signed statistical test.

Figure 2 Relative gene expression levels of TLR2 according to Helicobacter pylori infection. A: Comparison between Helicobacter pylori (H. pylori)-positive and H. pylori-negative groups, ^aP < 0.0001; H. pylori-positive or H. pylori-negative groups with samples of normal mucosa (relative quantification (RQ) = 1 represented for dashed line), ^bP < 0.0001 and P = 0.4305, respectively. B: Individual representation of RQ for each sample in H. pylori-positive group; C: Individual representation of RQ for each sample in H. pylori-negative group. ^{a, b}P < 0.05. RQ represented by median with interquartile range. Mann-Whitney and Wilcoxon Signed statistical test.

Figure 3 Relative gene expression levels of TLR2 according to polymorphic genotypes. A: Comparison between TLR2-196 to -174ins/ins (wild-type) and ins/del + del/del (polymorphic) in gastric cancer (GC), P = 0.0010 and chronic gastritis (CG), P = 0.5334 groups. B: TLR2-19216 TT (wild type) and TC + CC (polymorphic) in GC, P = 0.0004 and CG, P = 0.8827 groups. Significant difference, ^aP < 0.05. Relative quantification (RQ) represented by median with interquartile range. Mann-Whitney U statistical test.