Bai HX, Qiu XM, Xu CH, Guo JQ. MiRNA-145-5p inhibits gastric cancer progression via the serpin family E member 1- extracellular signal-regulated kinase-1/2 axis. World J Gastrointest Oncol 2024; 16(5): 2123-2140 [PMID: 38764835 DOI: 10.4251/wjgo.v16.i5.2123]
Corresponding Author of This Article
Jian-Qiang Guo, MD, PhD, Chief Physician, Director, Senior Researcher, Department of Gastroenterology, The Second Hospital of Shandong University, No. 247 Beiyuan Street, Tianqiao District, Jinan 250000, Shandong Province, China. guo_jianqiang@126.com
Research Domain of This Article
Oncology
Article-Type of This Article
Basic Study
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Bai HX, Qiu XM, Xu CH, Guo JQ. MiRNA-145-5p inhibits gastric cancer progression via the serpin family E member 1- extracellular signal-regulated kinase-1/2 axis. World J Gastrointest Oncol 2024; 16(5): 2123-2140 [PMID: 38764835 DOI: 10.4251/wjgo.v16.i5.2123]
Hong-Xia Bai, Jian-Qiang Guo, Department of Gastroenterology, The Second Hospital of Shandong University, Jinan 250000, Shandong Province, China
Hong-Xia Bai, Chun-Hong Xu, Department of Gastroenterology, Liaocheng People’s Hospital, Liaocheng 252000, Shandong Province, China
Xue-Mei Qiu, Department of Reproductive Center, Zaozhuang Maternal and Child Health Care Hospital, Zaozhuang 277000, Shandong Province, China
Author contributions: Bai HX and Qiu XM prepared material, data collection, analysis, and performed were design; Xu CH collected the samples; Bai HX and Xu CH performed animal experiments; Bai HX and Guo JQ wrote the manuscript. All authors read and approved the final manuscript. All authors contributed to the study’s conception and design.
Institutional review board statement: This study was reviewed and approved by the Ethics Committee of Liaocheng People's Hospital.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Liaocheng People's Hospital.
Informed consent statement: Written informed consent was obtained from all study participants, or their legal guardian included in this study.
Conflict-of-interest statement: The author(s) declared no potential conflicts of interest concerning the research, authorship, and/or publication of this article.
Data sharing statement: The authors confirm that the data supporting the findings of this study are available within the article.
ARRIVE guidelines statement: All the authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Corresponding author: Jian-Qiang Guo, MD, PhD, Chief Physician, Director, Senior Researcher, Department of Gastroenterology, The Second Hospital of Shandong University, No. 247 Beiyuan Street, Tianqiao District, Jinan 250000, Shandong Province, China. guo_jianqiang@126.com
Received: January 11, 2024 Peer-review started: January 11, 2024 First decision: January 30, 2024 Revised: February 19, 2024 Accepted: March 13, 2024 Article in press: March 13, 2024 Published online: May 15, 2024 Processing time: 118 Days and 22.3 Hours
ARTICLE HIGHLIGHTS
Research background
Gastric cancer (GC), a common malignancy of the digestive system, has an increasing incidence rate and poses a substantial threat to human health.
Research motivation
MicroRNAs (miRNA) play important roles in gene regulation and modulate many physical and pathological processes. The research motivation of this study was to identify the role of miRNA-145-5p (miR-145-5p) in the development of GC and its underlying mechanisms.
Research objectives
This study sought to compare miR-145-5p expression in GC and adjacent normal tissues, clarify the role of miR-145-5p in GC cell proliferation, invasion, metastasis, epithelial-mesenchymal transition (EMT), and apoptosis, and identify the direct target of miR-145-5p in GC cells.
Research methods
Real-time polymerase chain reaction was performed to detect miRNA expression. Wound-healing and Transwell assays were performed to evaluate cancer cell migration and invasion, respectively. Cell proliferation was examined using cell counting kit-8 and colony formation assays. Cell apoptosis was assessed using flow cytometry. Western blotting analysis was used to identify EMT-associated proteins. A dual-luciferase reporter system was used to validate the target of miR-145-5p. Tumor formation in nude mice was assessed to further explore the mechanism by which miR-145-5p inhibits the progression of GC.
Research results
MiR-145-5p was decreased in GC tissues. Serpin family E member 1 (SERPINE1) was characterized as a direct target of miR-363-3p. MiR-145-5p was shown to target SERPINE1 to regulate extracellular signal-regulated kinase-1/2 (ERK1/2) signaling.
Research conclusions
MiR-145-5p inhibits GC progression via the SERPINE1-ERK1/2 axis.
Research perspectives
The miR-145-5p/SERPINE1/ERK1/2 axis may serve as a GC treatment target.
