Published online Dec 15, 2022. doi: 10.4251/wjgo.v14.i12.2313
Peer-review started: July 14, 2022
First decision: September 26, 2022
Revised: October 17, 2022
Accepted: November 22, 2022
Article in press: November 22, 2022
Published online: December 15, 2022
Processing time: 151 Days and 4.4 Hours
In American colorectal cancer patients, the expression of N-myc downstream regulated gene 1 (NDRG1) in primary colorectal cancer was always less than that in adjacent normal tissues. Our study on clinical samples showed the opposite. Therefore, the effect of NDRG1 in cancer may be related to the ethnic backgrounds of colorectal cancer. The future research direction is to find out whether the role of NDRG1 in the development of colorectal tumors is related to ethnic differences through in vivo and in vitro experiments.
This study identifies the in vitro role of NDRG1 in Caucasian large intestine tumors as inhibition of tumor cell invasion and migration.
This study only showed the in vitro effect of NDRG1 in Caucasian large intestine tumors, and the question remains to be solved about the effect of NDRG1 in large intestine tumors of other races.
RNA extraction and quantitative polymerase chain reaction, western blotting, cell counting kit-8 assay, assessment of cell cycle by flow cytometric analysis, assessment of apoptosis by flow cytometric analysis, 24-transwell for invasion and migration were used in the experiments. GraphPad Prism 7.0. student’s t-test was used for statistical analysis.
The primary objective was to investigate the role of NDRG1 in the development and progression of colorectal tumors. The objective has been to investigate the in vitro role of NDRG1 in the development of Caucasian human bowel tumors. The significance of achieving these goals for future research in this area is to provide clinicians with a theoretical basis for selecting therapies for colorectal cancer.
The research topic is NDRG1 and the key problem to be solved is the role of NDRG1 in the occurrence and development of colorectal tumors. The results can provide a certain basis for the selection of treatment options for colorectal tumors.
In 2004, our research group published an article in the World Journal of Gastroenterology on our research data with a large clinical sample (150 cases): “Correlation of N-myc downstream-regulated gene 1 over-expression with progressive growth of colorectal neoplasm.”, which carried significance on the association of NDRG1 with colorectal cancer progression.