Overexpression of ELL-associated factor 2 suppresses invasion, migration, and angiogenesis in colorectal cancer

doi:10.4251/wjgo.v14.i10.1949

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 14, Issue 10

This Article

Peer-Review Report of This Article

CrossCheck and Google Search of This Article

Academic Rules and Norms of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (5952)

All Articles published online

The chart showing PDF series, WORD series, HTML series, Figures (1-9) series, Tables (1-3) series.

Item

Count

PDF

241

WORD

HTML

2031

Figures (1-9)

484

Tables (1-3)

507

Sum=3312

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

592

Download

2050

Sum=2642

Oct 15, 2022 (publication date) through Mar 2, 2026

Times Cited of This Article

Times Cited (5)

Journal Information of This Article

Publication Name

World Journal of Gastrointestinal Oncology

ISSN

1948-5204

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

- Full Article with Cover (PDF)
- Full Article (XML)

Basic Study

World J Gastrointest Oncol. Oct 15, 2022; 14(10): 1949-1967
Published online Oct 15, 2022. doi: 10.4251/wjgo.v14.i10.1949

Overexpression of ELL-associated factor 2 suppresses invasion, migration, and angiogenesis in colorectal cancer

Ming-Liang Feng, Can Wu, Hui-Jing Zhang, Huan Zhou, Tai-Wei Jiao, Meng-Yuan Liu, Ming-Jun Sun

Ming-Liang Feng, Can Wu, Hui-Jing Zhang, Huan Zhou, Tai-Wei Jiao, Meng-Yuan Liu, Ming-Jun Sun, Department of Endoscopy, The First Hospital Affiliated to China Medical University, Shenyang 110001, Liaoning Province, China

Author contributions: Feng ML and Wu C designed the research study; Zhang HJ and Jiao TW performed the research; Zhou H and Liu MY contributed new reagents and analytic tools; Feng ML and Sun MJ analyzed the data and wrote the manuscript; and all authors have read and approved the final manuscript.

Supported by the Natural Science Foundation of Liaoning Province, No. 2019-BS-279.

Institutional review board statement: This study was approved by the Institutional Review Board of The First Affiliated Hospital of China Medical University (Registration No. 2021-68-2).

Informed consent statement: Patients were not required to give informed consent to the study because the analysis used anonymous data that were obtained after each patient agreed to treatment by written consent.

Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.

Data sharing statement: No additional data are available.

Corresponding author: Ming-Jun Sun, Doctor, PhD, Chief Physician, Professor, Department of Endoscopy, The First Hospital Affiliated to China Medical University, No. 155 Nanjing North Street, Shenyang 110001, Liaoning Province, China. sunydyy@163.com

Received: May 19, 2022
Peer-review started: May 19, 2022
First decision: June 6, 2022
Revised: June 20, 2022
Accepted: September 21, 2022
Article in press: September 21, 2022
Published online: October 15, 2022
Processing time: 147 Days and 20.6 Hours

ARTICLE HIGHLIGHTS

Research background

There are few studies on the expression and role of ELL-associated factor 2 (EAF2) protein in colorectal cancer (CRC). The molecular mechanism of involvement of EAF2 in CRC invasion and metastasis remains unclear.

Research motivation

EAF2 may play a tumor suppressive and protective role in the development of CRC by inhibiting the invasion, metastasis, and angiogenesis of CRC cells.

Research objectives

We assessed the expression and localization of EAF2 protein in CRC specimens. Meanwhile, we cultured CRC cells in vitro to determine the expression of EAF2 protein and further investigate its effects on the biological function of CRC cells.

Research methods

We used immunohistochemistry and western blot assay to detect the expression of EAF2 protein in CRC tissues from 70 patients. In addition, we applied plasmid transfection technology to study the effects of EAF2 on the invasion, migration, and angiogenesis of CRC cells in vitro.

Research results

EAF2 protein was lowly expressed in cancer tissues of patients with advanced CRC. Kaplan-Meier survival analysis showed that the survival rate of the group with high EAF2 level was higher than that of the group with low EAF2 level. In addition, EAF2 affected the biological functions of CRC cells by regulating the STAT3/TGF-β1 crosstalk pathway.

Research conclusions

As a tumor suppressor, EAF2 may play a tumor suppressive and protective role in the development of CRC by inhibiting the invasion, metastasis, and angiogenesis of CRC cells via regulating the signal transducer and activator of transcription 3/transforming growth factor beta 1 crosstalk pathway. These findings may provide new diagnostic markers and novel therapeutic targets for CRC.

Research perspectives

Our data is beneficial to elucidate the molecular mechanism of CRC invasion and metastasis as well as explore new potential molecular markers and the direction of targeted therapy.