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©The Author(s) 2024. Published by Baishideng Publishing Group Inc. All rights reserved.
Dysbiosis promotes recurrence of adenomatous polyps in the distal colorectum
Li-Li Yin, Ping-Qian Qi, Yun-Fei Hu, Xiao-Jun Fu, Rui-Shan He, Meng-Meng Wang, Yan-Juan Deng, Su-Yi Xiong, Qi-Wen Yu, Jin-Ping Hu, Lv Zhou, Zhi-Bin Zhou, Ying Xiong, Huan Deng
Li-Li Yin, Ping-Qian Qi, Yun-Fei Hu, Xiao-Jun Fu, Meng-Meng Wang, Yan-Juan Deng, Su-Yi Xiong, Qi-Wen Yu, Jin-Ping Hu, Lv Zhou, Zhi-Bin Zhou, Huan Deng, The Affiliated Rehabilitation Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, Jiangxi Province, China
Li-Li Yin, Ping-Qian Qi, Yun-Fei Hu, Xiao-Jun Fu, Meng-Meng Wang, Yan-Juan Deng, Su-Yi Xiong, Qi-Wen Yu, Jin-Ping Hu, Lv Zhou, Zhi-Bin Zhou, Huan Deng, The Fourth Affiliated Hospital, Jiangxi Medical College, Nanchang University, Nanchang 330006, Jiangxi Province, China
Rui-Shan He, The Second College of Clinical Medicine, Jiangxi Medical College, Nanchang University, Nanchang 330006, Jiangxi Province, China
Meng-Meng Wang, Yan-Juan Deng, Su-Yi Xiong, Qi-Wen Yu, Jin-Ping Hu, Lv Zhou, Zhi-Bin Zhou, Huan Deng, Tumor Immunology Institute, Nanchang University, Nanchang 330006, Jiangxi Province, China
Ying Xiong, Department of General Medicine, The Second College of Clinical Medicine, Jiangxi Medical College, Nanchang University, Nanchang 330031, Jiangxi Province, China
Huan Deng, The Ministry of Education Basic Research and Innovation Center for the Targeted Therapeutics of Solid Tumors, Jiangxi Medical College, Nanchang University, Nanchang 330031, Jiangxi Province, China
Co-corresponding authors: Ying Xiong and Huan Deng.
Author contributions: Yin LL, Fu XJ, Xiong Y, and Deng H designed the research study; Yin LL, Qi PQ, Hu YF, Fu XJ, He RS, and Deng H wrote the manuscript; Yin LL, Qi PQ, Hu YF, Fu XJ, He RS, Wang MM, Deng YJ, Xiong SY, Yu QW, Hu JP, Zhou L, and Zhou ZB collected the clinical data and pathological photographs; Yin LL analyzed the data and drew the figures; and all authors have read and approved the final manuscript. Xiong Y and Deng H contributed equally to this study as co-corresponding authors.
Supported by National Science Foundation of China, No. 82160546; the Science Foundation of Jiangxi Province, No. 20202BBG73027; the Foundation of Jiangxi Province for Distinguished Scholars, No. jxsq2023201020; and the Science and Technology Project of Jiangxi Administration of Traditional Chinese Medicine, No. 2022B789.
Institutional review board statement: This study was conducted in accordance with the Declaration of Helsinki and approved by the Ethics Committee of the Fourth Affiliated Hospital of Nanchang University (No. SFYYXLL-PJ-2021-KY018). Each patient provided written informed consent for the collection of samples and subsequent analysis.
Institutional animal care and use committee statement: All mouse studies were approved by the Fourth Affiliated Hospital of Nanchang University Institutional Animal Care and Use Committee (No. 2017-024).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
Data sharing statement: The original contributions presented in the study are included in the article (
Supplementary material), further inquiries can be directed to the corresponding author.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See:
https://creativecommons.org/Licenses/by-nc/4.0/ Corresponding author: Huan Deng, MD, PhD, Professor, The Affiliated Rehabilitation Hospital, Jiangxi Medical College, Nanchang University, No. 133 South Guangchang Road, Nanchang 330006, Jiangxi Province, China.
beandeng@ncu.edu.cn
Received: February 14, 2024
Revised: May 19, 2024
Accepted: June 14, 2024
Published online: August 15, 2024
Processing time: 175 Days and 13.5 Hours
BACKGROUND
Colorectal polyps, which are characterized by a high recurrence rate, represent preneoplastic conditions of the intestine. Due to unclear mechanisms of pathogenesis, first-line therapies for non-hereditary recurrent colorectal polyps are limited to endoscopic resection. Although recent studies suggest a mechanistic link between intestinal dysbiosis and polyps, the exact compositions and roles of bacteria in the mucosa around the lesions, rather than feces, remain unsettled.
AIM
To clarify the composition and diversity of bacteria in the mucosa surrounding or 10 cm distal to recurrent intestinal polyps.
METHODS
Mucosal samples were collected from four patients consistently with adenomatous polyps (Ade), seven consistently with non-Ade (Pol), ten with current Pol but previous Ade, and six healthy individuals, and bacterial patterns were evaluated by 16S rDNA sequencing. Linear discriminant analysis and Student’s t-tests were used to identify the genus-level bacteria differences between groups with different colorectal polyp phenotypes. Pearson’s correlation coefficients were used to evaluate the correlation between intestinal bacteria at the genus level and clinical indicators.
RESULTS
The results confirmed a decreased level of probiotics and an enrichment of pathogenic bacteria in patients with all types of polyps compared to healthy individuals. These changes were not restricted to the mucosa within 0.5 cm adjacent to the polyps, but also existed in histologically normal tissue 10 cm distal from the lesions. Significant differences in bacterial diversity were observed in the mucosa from individuals with normal conditions, Pol, and Ade. Increased abundance of Gram-negative bacteria, including Klebsiella, Plesiomonas, and Cronobacter, was observed in Pol group and Ade group, suggesting that resistance to antibiotics may be one risk factor for bacterium-related harmful environment. Meanwhile, age and gender were linked to bacteria changes, indicating the potential involvement of sex hormones.
CONCLUSION
These preliminary results support intestinal dysbiosis as an important risk factor for recurrent polyps, especially adenoma. Targeting specific pathogenic bacteria may attenuate the recurrence of polyps.
Core Tip: This study investigates the links between intestinal dysbiosis and recurrent colorectal polyps using 16S rDNA sequencing. The findings reveal reduced probiotics and increased pathogenic bacteria in the mucosa surrounding or 10 cm distal to the polyps. Significant differences in bacterial diversity were found among the normal mucosa, non-adenomous polyps, and adenomous polyps. Increased Gram-negative bacteria, such as Klebsiella, Plesiomonas, and Cronobacter, suggest that antibiotic resistance may contribute to the establishment of a harmful environment. Age and gender also influence bacterial changes, indicating the involvement of sex hormones. Targeting specific pathogenic bacteria may facilitate the prevention of recurrent polyps.
