Ghosh M, Nandi S, Dutta S, Saha MK. Detection of hepatitis B virus infection: A systematic review. World J Hepatol 2015; 7(23): 2482-2491 [PMID: 26483870 DOI: 10.4254/wjh.v7.i23.2482]
Corresponding Author of This Article
Malay Kumar Saha, PhD, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme XM, Beleghata, Kolkata 700010, India. sahamk@yahoo.com
Research Domain of This Article
Infectious Diseases
Article-Type of This Article
Systematic Reviews
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Hepatol. Oct 18, 2015; 7(23): 2482-2491 Published online Oct 18, 2015. doi: 10.4254/wjh.v7.i23.2482
Detection of hepatitis B virus infection: A systematic review
Mallika Ghosh, Srijita Nandi, Shrinwanti Dutta, Malay Kumar Saha
Mallika Ghosh, Srijita Nandi, Shrinwanti Dutta, Malay Kumar Saha, National Institute of Cholera and Enteric Diseases, Kolkata 700010, India
Author contributions: Ghosh M, Nandi S, Dutta S and Saha MK contributed equally to the work; all conceptualized and designed the review, drafted the manuscript, reviewed and approved the final manuscript as submitted.
Conflict-of-interest statement: All the authors declare that they have no competing interest.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at sahamk@yahoo.com. No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Malay Kumar Saha, PhD, National Institute of Cholera and Enteric Diseases, P-33, C.I.T. Road, Scheme XM, Beleghata, Kolkata 700010, India. sahamk@yahoo.com
Telephone: +91-94-33081013 Fax: +91-33-23632398
Received: April 28, 2015 Peer-review started: May 6, 2015 First decision: July 29, 2015 Revised: August 18, 2015 Accepted: September 29, 2015 Article in press: September 30, 2015 Published online: October 18, 2015 Processing time: 173 Days and 20.5 Hours
Core Tip
Core tip: The article was aimed to review published methods of detection of hepatitis B virus (HBV) infection. A thorough search on medline database was conducted and 72 studies were included. It was observed that HBV can be detected reliably from dried blood spot (sensitivity > 90%). Serological and Molecular assays are predominant and reliable methods. Chemiluminescent immunoassay is more sensitive than Enzyme linked immunosorbent assay. Rapid tests are useful for screening. Real time polymerase chain reaction (PCR), branched DNA probe assays are principal methods for quantitation. Automated systems are more sensitive compared to in house assays. Abbott real time PCR was found to be most sensitive with a lower detection limit of only 1.48 IU/mL.
