Induction of CXC chemokines in human mesenchymal stem cells by stimulation with secreted frizzled-related proteins through non-canonical Wnt signaling

Table 1 siRNA oligonucleotide sequences

Gene	Qiagen product name	Qiagen catalog No.	Human target sequence
siFZD2	Hs_FZD2_5	SI02757433	CACGGTCTACATGATCAAATA
siFZD5	Hs_FZD5_5	SI02757650	TAAGGTTGGCGTTGTAATGAA
siROR2	Hs_ROR2_6	SI00287525	CTGGTGCTTTACGCAGAATAA
siScambled	Ctrl_Control_1	SI03650325	AATTCTCCGAACGTGTCACGT

Table 2 Reverse transcription-polymerase chain reaction primer sequences

Gene	Human primer sequence
hCXCL5	5’GCTGGTCCTGCCGCTGCTGTG3’
	5’GTTTTCCTTGTTTCCACCGTC3’
hCXCL8	5’GCCTTCCTGATTTCTGCAGC3’
	5’TCCAGACAGAGCTCTCTTCC3’
18S RibosomalRNA	5’CGGGTCATAAGCTTGCGTT3’
	5’CCGCAGGTTCACCTACGG3’
FZD2	5’CCTCAAGGTGCCATCCTATCTCAG3’
	5’GTGTAGCAGCCCGACAGAAAAATG3’
FZD5	5’CCTACCACAAGCAGGTGTCC3’
	5’GGACAGGTTCTTCCTCGAAA3’
ROR2	5’TCCTTCTGCCACTTCGTGTTTCC3’
	5’TGCTTGCCGTTCCTCTGTAATCC3’