Induction of CXC chemokines in human mesenchymal stem cells by stimulation with secreted frizzled-related proteins through non-canonical Wnt signaling

doi:10.4252/wjsc.v7.i11.1262

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Dec 26, 2015; 7(11): 1262-1273
Published online Dec 26, 2015. doi: 10.4252/wjsc.v7.i11.1262

Table 1 siRNA oligonucleotide sequences

Gene	Qiagen product name	Qiagen catalog No.	Human target sequence
siFZD2	Hs_FZD2_5	SI02757433	CACGGTCTACATGATCAAATA
siFZD5	Hs_FZD5_5	SI02757650	TAAGGTTGGCGTTGTAATGAA
siROR2	Hs_ROR2_6	SI00287525	CTGGTGCTTTACGCAGAATAA
siScambled	Ctrl_Control_1	SI03650325	AATTCTCCGAACGTGTCACGT

Full Size Table

Table 2 Reverse transcription-polymerase chain reaction primer sequences

Gene	Human primer sequence
hCXCL5	5’GCTGGTCCTGCCGCTGCTGTG3’
hCXCL5	5’GTTTTCCTTGTTTCCACCGTC3’
hCXCL8	5’GCCTTCCTGATTTCTGCAGC3’
hCXCL8	5’TCCAGACAGAGCTCTCTTCC3’
18S RibosomalRNA	5’CGGGTCATAAGCTTGCGTT3’
18S RibosomalRNA	5’CCGCAGGTTCACCTACGG3’
FZD2	5’CCTCAAGGTGCCATCCTATCTCAG3’
FZD2	5’GTGTAGCAGCCCGACAGAAAAATG3’
FZD5	5’CCTACCACAAGCAGGTGTCC3’
FZD5	5’GGACAGGTTCTTCCTCGAAA3’
ROR2	5’TCCTTCTGCCACTTCGTGTTTCC3’
ROR2	5’TGCTTGCCGTTCCTCTGTAATCC3’

Full Size Table

Citation: Bischoff DS, Zhu JH, Makhijani NS, Yamaguchi DT. Induction of CXC chemokines in human mesenchymal stem cells by stimulation with secreted frizzled-related proteins through non-canonical Wnt signaling. World J Stem Cells 2015; 7(11): 1262-1273
URL: https://www.wjgnet.com/1948-0210/full/v7/i11/1262.htm
DOI: https://dx.doi.org/10.4252/wjsc.v7.i11.1262