Neural stem cells isolated from amyloid precursor protein-mutated mice for drug discovery

Table 1 Primary and secondary antibodies used in the study

Antibody		Specie	Supplier	Dilution
Primary antibody
	β-III-Tubulin (TuJ-1)	Mouse	R and D	1:1000
	GFAP	Rabbit	Eurodiagnostic	1:100
	MAP2	Rabbit	S.Cruz	1:250
	6E10	Mouse	Covance	1:1000
	CNPase	Mouse	Chemicon	1:250
Secondary antibody
	Anti-Mo RRX	Donkey	Alexa	1:600
	Anti-Mo Cy2	Donkey	Jackson	1:100
	Anti-Rb Cy2	Donkey	Jackson	1:100
	Anti-Rb RRX	Donkey	Jackson	1:100

The antibodies used, the species in which they were produced, the manufacturers and the dilutions at which they were used are described in the table. RRX: Rhodamine red-x conjugated.

Table 2 Genotype-dependent cell yield per animal

	Wild type	Tg2576
Primary neurospheres	1.7 × 105± 5 × 104	1.7 × 105± 1×104
Secondary neurospheres	1.7 × 105± 1.4× 104	1.3×105± 9.7×103a

Cells obtained from 4 animals were counted in primary and secondary neurospheres development. A decrease in the total number of cells per animal in Tg2576-compared to Wt culture was observed, ^aP < 0.05. Statistical studies performed by Student’s t-test.

Table 3 Polymerase chain reaction array analysis

Gene	Regulation
Acetylcholinesterase	3.41
Amyloid beta (A4) precursor protein-binding, family B, member 1	2.33
CDK5 regulatory subunit associated protein 1	2.07
Dopamine receptor D2	2.62
V-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)	2.17
Glial cell line derived neurotrophic factor	3.13
Interleukin 3	2.69
Notch gene homolog 2 (Drosophila)	2.29
Odd Oz/ten-m homolog 1 (Drosophila)	3.81
Par-3 (partitioning defective 3) homolog (C. elegans)	2.39
Vascular endothelial growth factor A	3.01

Table represent the 11 genes resulted differentially expressed in Tg2576 neural stem cells (NSCs) related to Wt cells. Regulation is indicated as folds of increase. All the included differentially expressed genes resulted overexpressed in Tg2576 cells.