Insulin producing cells established using non-integrated lentiviral vector harboring PDX1 gene

Table 1 Sequences of primers used in real-time reverse transcription-polymerase chain reaction and polymerase chain reaction experiments

Gene	Gene ID	Size (bp)	Strand	Sequence (5'–3')	Annealing temperature
Somatostatin	NM_001048.3	114	F	GCACCCCGAGAACGCAAAGC	56
			R	TGTGGGGGCGAGGGATCAGAG
Ngn3	NM_020999.3	241	F	GTAGAAAGGATGACGCCTCAACC	54
			R	TCAGTGCCAACTCGCTCTTAGG
Glucagon	NM_002054.4	187	F	CAGTGCGCCTTGGTGCAGAAGT	58
			R	GGAACGTTGCCAGCTGCCTTG
Glut2	NM_000340	300	F	AGCTTTGCAGTTGGTGGAAT	52
			R	AATAAGAATGCCCGTGACGA
PDX1	NM_000209.3	201	F	GTCCGGTGCCAGAGTTCAGT	63
			R	CCCAGTCTCGGTTCCATTCG
β-actin	NM_001101.3	161	F	GAGACCTTCAACACCCCAGCC	56
			R	AGACGCAGGATGGCATGGG

Ngn3: neurogenin3; Glut2: glucose transporter2.