miR-140-3p in exosomes derived from bone-marrow mesenchymal stem cells inhibits pyrotoisosis by targeting PTEN under high-glucose condition

Table 1 Primer sequences

Primer		Gene	Primer sequences (5’-3’)
R-U6	RT	K00784.1	AACGCTTCACGAATTTGCGT
	Forward		CCTGCTTCGGCAGCACAT
	Reverse		AACGCTTCACGAATTTGCGT
miR-140-3p	RT	MIMAT0000574	CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCCGTGGTT
	Forward		TACCACAGGGTAGAACCACGG
	Reverse		CTCAACTGGTGTCGTGGAGTC

Table 2 Antibody information

Name of antibody	Antibody dilution	Company	Catalog number
GSDMD-N	1:1000	Abclonal	A22523
Cleaved caspase-1	1:500	Affbiotech	AF4005
IL-1β	1:2000	Proteintech Group	26048-1-AP
IL-18	1:1000	Abcam	ab191860
NLRP3	1:1000	Proteintech Group	68102-1-Ig
CD9	1:1000	Proteintech Group	20597-1-AP
CD63	1:500	Affbiotech	AF5117
CD81	1:1000	Abcam	ab109201

GSDMD-N: Gasdermine-D-N-terminal; IL: Interleukin.

Table 3 The primer sequences

Primer	Primer sequences (5’-3’)		Tm	CG%
R-ACTIN	Sense	CGTTGACATCCGTAAAGACCTC	58.4	52.4
	Antisense	TAGGAGCCAGGGCAGTAATCT	59.9	52.4
R-PTEN	Sense	CTCAGCCATTGCCTGTGTGT	59	55
	Antisense	TCCTTGTCATTATCCGCACG	59.3	50

PTEN: Phosphatase and tensin homolog.