Sclerostin-silenced human umbilical cord mesenchymal stem cells ameliorate bone metabolism in steroid-induced femoral head necrosis

doi:10.4252/wjsc.v17.i10.110190

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Oct 26, 2025 (publication date) through Oct 27, 2025

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Oct 26, 2025; 17(10): 110190
Published online Oct 26, 2025. doi: 10.4252/wjsc.v17.i10.110190

Table 1 Sclerostin gene silencing target sequence

Target		Sequence
mSOST-shR1	F	5’-GATCCGCCAGTCGGAGCTCAAGGACTTTCAAGAGAGTCCTTGAGCTCCGACTGGCTTTTTACGCGTG-3’
mSOST-shR1	R	5’-AATTCACGCGTAAAAAACCAGTCGGAGCTCAAGGACTCTCTTGAAAGTCCTTGAGCTCCGACTGGCG-3’
mSOST-shR2	F	5’-GATCCGGCCTTCAGGAATGATGCCACATTCAAGAGTGTGGCATCATTCCTGAAGGCTTTTTTACGCGTG-3’
mSOST-shR2	R	5’-AATTCACGCGTAAAAAAGCCTTCAGGAATGATGCCACACTCTTGAATGTGGCATCATTCCTGAAGGCCG-3’

SOST: Sclerostin.

Full Size Table

Table 2 Primers used for quantitative real-time polymerase chain reaction of total RNA

Genes	Primers types	Sequence (5’-3’)
ALP	Forward	TGTGGAAGGCTCTGGAAAGT
ALP	Reverse	TGTCAACAGGATCCAGGCAT
OPG	Forward	ACCCCAGAGCGAAATACAGT
OPG	Reverse	TGTCGTGTGTTGCATTTCCT
Osterix	Forward	CAAGGTGTATGGCAAGGCTT
Osterix	Reverse	GCAGGCAGGTGAACTTCTTC
SOX9	Forward	GAACAAGCCGCACGTCAA
SOX9	Reverse	CGCGGCTGGTACTTGTAATC
COL2A1	Forward	TCGTGGTGACAAAGGTGAAA
COL2A1	Reverse	CCAGCCTTTTCATCAAATCC
ACAN	Forward	ACAAGGTCTCACTGCCCAAC
ACAN	Reverse	AAGTCGAGGGTGTAGCGTGT
PPAR/γ	Forward	GCCAAGAACATCCCCAACTTC
PPAR/γ	Reverse	GCAAAGATGGCCTCATGCA
C/EBPs	Forward	CCGAGAATGGGAAGCTTGTC
C/EBPs	Reverse	AAGCACCAACGAGAGGAGAA
AP2	Forward	GGGTGTCGATGCTGACATTA
AP2	Reverse	TACTCTGGCGACGAAGTATTTG
SOST	Forward	AGAACAACAAGACCATGAAC
SOST	Reverse	GCGGCACGGCCCATCGGTC
GADPH	Forward	CCATCTTCCAGGAGCGAGAT
GADPH	Reverse	GGTCATGAGTCCTTCCACGA

ALP: Alkaline phosphatase; OPG: Osteoprotegerin; SOX9: SRY-box transcription factor 9; COL2A1: Type II collagen alpha 1 chain; ACAN: Aggrecan; PPAR/γ: Peroxisome proliferator-activated receptor gamma; C/EBPs: CCAAT/enhancer-binding proteins; AP2: Adipocyte protein 2; SOST: Sclerostin; GADPH: Glyceraldehyde-3-phosphate dehydrogenase.

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Citation: Lv H, Zheng CF, Chen XY, Wei JH, Tao YZ, Feng L, Feng Z, Lu SJ. Sclerostin-silenced human umbilical cord mesenchymal stem cells ameliorate bone metabolism in steroid-induced femoral head necrosis. World J Stem Cells 2025; 17(10): 110190
URL: https://www.wjgnet.com/1948-0210/full/v17/i10/110190.htm
DOI: https://dx.doi.org/10.4252/wjsc.v17.i10.110190