Zinc enhances the cell adhesion, migration, and self-renewal potential of human umbilical cord derived mesenchymal stem cells

Table 1 Primers for cell cycle and proliferation genes

S. No	Genes	Primer sequence (5’-3’)	Annealing temperature (ºC)
1	GAPDH	(F) CCAGAACATCATCCCTGCCT	58
		(R) CCTGCTTCACCACCTTCTTG
2	CDC20	(F) TCCTGAGTCTGACCATGAGC	58
		(R) CTGAGGTGATGGGTTGGTCT
3	CDK1	(F) GGGGTCAGCTCGTTACTCAA	58
		(R) CACTTCTGGCCACACTTCAT
4	CCNA2	(F) CCAGGAGAATATCAACCCGGA	58
		(R) GGAACGGTGACATGCTCATC
5	CDCA2	(F) TCTGGGAGTAAGCCTGTCTG	58
		(R) AGGAGGTAATTCGGCATGCT
6	TGFβ1	(F) CAAGGCACAGGGGACCAG	58
		(R) CAGGTTCCTGGTGGGCAG
7	GDF5	(F) CACATCCCAAGAGCCCCTTC	58
		(R) GCCCAGGTGAGGAGAAATGG
8	HIF1α	(F) CTCGAGATGCAGCCAGATCT	58
		(R) CCAGAAGTTTCCTCACACGC
9	45203	(F) GATGTCAGGGCTCTTTGTCCA	54
		(R) TACTCTCCCCAGCTTGCTTTG
10	SOX-2	(F) CCCTGCAGTACAACTCCATGAC	54
		(R) GGTAGTGCTGGGACATGTGAAG
11	NANOG	(F) AAGGCAAACAACCCACTTCTG	54
		(R) CCAGTTGTTTTTCTGCCACCT
12	CXCR 4	(F) ACTGAGAAGCATGACGGACA	54
		(R) TCATCTGCCTCACTGACGTT
13	VCAM1	(F) CCTGGGAAGATGGTCGTGAT	54
		(R) AGATGTGGTCCCCTCATTCG
14	VEGF-A	(F) AGCGCAAGAAATCCCGGT	54
		(R) CCCTCCGGACCCAAAGTG
15	MMP2	(F) TTGACGGTAAGGACGGACTC	54
		(R) AGCGGAATGGAAACTTGCAG

Housekeeping gene: GAPDH (Glyceraldehyde-3 Phosphate Dehydrogenase); cell cycle genes: CDC20 (Cell-Division Cycle Protein 20), CDK1 (Cyclin-Dependent Kinase 1), CCNA2 (Cyclin-A2), CDCA2 (Cell Division Cycle Associated 2); proliferation genes: TGFβ (Transforming Growth Factor β1), 1, GDF5 (Growth Differentiation Factor 5), HIF1α (Hypoxia-Inducible Factor 1α); pluripotency genes: OCT-4 (Octamer-Binding Transcription Factor-4), SOX2 (SRY-box 2), NANOG; migration genes: CXCR 4 (Chemokine Receptor Type 4), VCAM1 (Vascular Cell Adhesion Molecule1), VEGF-A (Vascular Endothelial Growth Factor-A), MMP2 (Matrix Metallopeptidase 2).