Bone marrow mesenchymal stem cell-derived exosomal microRNAs target PI3K/Akt signaling pathway to promote the activation of fibroblasts

Table 1 Primers used in the experiment

Genes	Primers (5’ - 3’)
PTEN	F: TCAGCCGTTACCTGTGTGTG
	R: TCCTTGTCATTATCTGCACGC
α-SMA	F: GACAATGGCTCTGGGCTCTGTAA
	R: CTGTGCTTCGTCACCCACGTA
Vimentin	F: GACGCCATCAACACCGAGTT
	R: CTTTGTCGTTGGTTAGCTGGT
Tenomodulin	F: CCATGCTGGATGAGAGAGGTT
	R: CCGTCCTCCTTGGTAGCAGT
Col I	F: CCCGGGTTTCAGAGACAACTTC
	R: TCCACATGCTTTATTCCAGCAATC
Sox 9	F: GAGCCGGATCTGAAGAGGGA
	R: GCTTGACGTGTGGCTTGTTC
Col II	F: GGGAATGTCCTCTGCGATGAC
	R: GAAGGGGATCTCGGGGTTG
GAPDH	F: ACTCCACTCACGGCAAATTC
	R: TCTCCATGGTGGTGAAGACA

PTEN: Phosphatase and tensin homolog; α-SMA: α-smooth muscle actin positive.

Table 2 Top 10 clusters with Gene Ontology Biological Processes analysis of hub-gene targets

Term	Description	LogP	Log(q-value)	Count
GO:0048732	Gland development	-12.279	-7.927	9
GO:0048608	Reproductive structure development	-10.269	-6.495	8
GO:0061458	Reproductive system development	-10.245	-6.495	8
GO:0030900	Forebrain development	-8.922	-5.472	7
GO:0043408	Regulation of MAPK cascade	-8.603	-5.240	8
GO:0045596	Negative regulation of cell differentiation	-8.549	-5.238	8
GO:0022612	Gland morphogenesis	-8.482	-5.208	5
GO:0014066	Regulation of phosphatidylinositol 3-kinase signaling	-8.316	-5.077	5
GO:0060322	Head development	-8.138	-4.931	8
GO:0060284	Regulation of cell development	-8.045	-4.868	7

Table 3 Top 10 clusters with Gene Ontology Cellular Components analysis of hub-gene targets

Term	Description	LogP	Log(q-value)	Count
GO:0045121	Membrane raft	-4.441	-2.311	4
GO:0098857	Membrane microdomain	-4.436	-2.310	4
GO:0001650	Fibrillar center	-4.076	-2.035	3
GO:0009898	Cytoplasmic side of plasma membrane	-3.849	-1.860	3
GO:0098562	Cytoplasmic side of membrane	-3.638	-1.688	3
GO:0005770	Late endosome	-3.205	-1.308	3
GO:0005788	Endoplasmic reticulum lumen	-3.103	-1.227	3
GO:0031983	Vesicle lumen	-3.041	-1.174	3
GO:0005925	Focal adhesion	-2.726	-0.896	3
GO:0030055	Cell-substrate junction	-2.699	-0.873	3

Table 4 Top 10 clusters with Gene Ontology Molecular Functions analysis of hub-gene targets

Term	Description	LogP	Log(q-value)	Count
GO:0002020	Protease binding	-5.941	-3.326	4
GO:0004712	Protein serine/threonine /tyrosine kinase activity	-5.299	-2.910	5
GO:0004672	Protein kinase activity	-4.802	-2.546	5
GO:0003682	Chromatin binding	-4.726	-2.507	5
GO:0008134	Transcription factor binding	-4.694	-2.487	5
GO:0031490	Chromatin DNA binding	-4.457	-2.319	3
GO:0016773	Phosphotransferase activity, alcohol group as acceptor	-4.435	-2.310	5
GO:0019904	Protein domain specific binding	-4.395	-2.280	5
GO:0061629	RNA polymerase II-specific DNA-binding transcription factor binding	-4.331	-2.228	4
GO:0016301	Kinase activity	-4.267	-2.193	5

Table 5 Top 10 clusters with Kyoto Encyclopedia of Genes and Genomes Pathway analysis of hub-gene targets

Term	Description	LogP	Log(q-value)	Count
hsa04510	Focal adhesion	-10.807	-6.755	7
hsa05200	Pathways in cancer	-9.488	-5.834	8
hsa04151	PI3K-Akt signaling pathway	-9.088	-5.538	7
hsa01521	EGFR tyrosine kinase inhibitor resistance	-9.046	-5.538	5
hsa05215	Prostate cancer	-8.593	-5.240	5
hsa05224	Breast cancer	-7.682	-4.608	5
hsa05165	Human papillomavirus infection	-7.560	-4.511	6
hsa05213	Endometrial cancer	-7.435	-4.444	4
hsa05225	Hepatocellular carcinoma	-7.392	-4.437	5
hsa05230	Central carbon metabolism in cancer	-7.102	-4.181	4

EGFR: Epidermal growth factor receptor.