Disease modifying treatment of spinal cord injury with directly reprogrammed neural precursor cells in non-human primates

Table 1 Characteristics of experimental groups

Group	No/Age1/Weight2	First surgery (Week 0)	Second surgery (2 wk after the injury)	Hindlimb score	MRI	SSEPs, MEPs	Histology and IHC
Lesion control	LC1/4.3/5.2 LC2/4.2/5.3 LC3/4.1/5.1	Resection of 25% area of spinal cord in the projections of lateral pyramidal tracts and the dorsal column at the level Th 7-8 (as described)	Sham surgery + vehicle	Before transplant and 2, 4, 6, 8, and 12 wk post transplant	Before transplant and 2, 4, 8, and 12 wk post transplant	Before lesion, (intraoperatively, D0), 2 wk after lesion (D14), 8 and 12 wk post transplant	Nissl, van Gieso; IHC: GFAP, NF200, BDNF
NPC	NPC1/3.3/4.1 NPC2/4.3/4.6 NPC3/3.8/4.6 NPC4/3.6/4.1		Implantation of 5 × 106 drNPCs

¹Age in years.

²Weight in kilograms at the beginning of the experiment. BDNF: Brain derived neurotrophic factor; drNPCs: Directly reprogrammed neural precursor cells; GFAP: Glial fibrillary acidic protein; IHC: Immunohistochemistry; MEP: Motor evoked potential; MRI: Magnetic resonance imaging; NPC: Neural progenitor cell group; SSEP: Somatosensory evoked potential.