Bone marrow mesenchymal stem cells induce M2 microglia polarization through PDGF-AA/MANF signaling

doi:10.4252/wjsc.v12.i7.633

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Jul 26, 2020 (publication date) through Oct 22, 2025

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World Journal of Stem Cells

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World J Stem Cells. Jul 26, 2020; 12(7): 633-658
Published online Jul 26, 2020. doi: 10.4252/wjsc.v12.i7.633

Table 1 Primers used for real-time quantitative reverse transcription polymerase chain reaction analysis

Gene	Forward primer (5'–3')	Reverse primer (5'–3')
MANF	TCCGCTACTGTAAGCAAGGT	CTTCACCTAGGATCTTGGTG
PDGF-AA	GCCATTCCCGCAGTTTG	GGCTGGCACTTGACGCT
XBP1	ATGTTTTTCAAATGTCCTTCCCCAG	TGACAGAGAAAGGGAGGCTGGTAAG
ATF6	TGCAGGTGTATTACGCTTCGC	GCAGGTGATCCCTTCGAAATG
iNOS	ATCCCGAAACGCTACACTT	CGGCTGGACTTCTCACTC
Arg1	CAGTGGCGTTGACCTTGT	TGGTTCTGTTCGGTTTGC
NF-YA	CTGAGACTCCACAGCCATCA	GGATCTTCCCTTCTGCCTCT
NF-YB	CTGGATGGGGCTGACTGTAT	AGGAGGTACGCCAGTCTGTG
NF-YC	ACCCTTGCATGGCACTAAAG	GCTGGATGGTGGTCGTAGAA
miR-30a*	CTTTCAGTCGGATGTTTGC	GTGCGTGTCGTGGAGTCG
GAPDH	GCAAGTTCAACGGCACAG	GCCAGTAGACTCCACGACAT
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT

MANF: Mesencephalic astrocyte–derived neurotrophic factor; PDGF-AA: Platelet-derived growth factor-AA; XBP1: X-box binding protein 1; ATF6: Activating transcription factor 6; iNOS: Inducible nitric oxide synthase; Arg-1: Arginase-1; NF-Y: Nuclear factor-Y; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.

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Citation: Yang F, Li WB, Qu YW, Gao JX, Tang YS, Wang DJ, Pan YJ. Bone marrow mesenchymal stem cells induce M2 microglia polarization through PDGF-AA/MANF signaling. World J Stem Cells 2020; 12(7): 633-658
URL: https://www.wjgnet.com/1948-0210/full/v12/i7/633.htm
DOI: https://dx.doi.org/10.4252/wjsc.v12.i7.633