Safety of menstrual blood-derived stromal cell transplantation in treatment of intrauterine adhesion

Table 1 Body weight changes of mice in toxicity study

	Group 1	Group 2	Group 3	Group 4	Group 5	Group 6
Body weight (g)	228.0 ± 18.18	228.8 ± 9.175	267.4 ± 26.16	265.8 ± 9.378	326.0 ± 22.75	303.7 ± 35.06

Measurements are given as the mean ± SD. Group 1: Control for acute group; Group 2: Acute group; Group 3: Control for subchronic group; Group 4: Subchronic group; Group 5: Control for chronic group; Group 6: Chronic group (n = 5 for each control group, n = 10 for each experimental group).

Table 2 Relative organ weights of rats in toxicity study

	Group 1	Group 2	Group 3	Group 4	Group 5	Group 6
Brain	1.314 ± 0.220	1.439 ± 0.176	1.480 ± 0.211	1.477 ± 0.197	1.441 ± 0.047	1.508 ± 0.176
Heart	0.718 ± 0.048	0.750 ± 0.087	0.744 ± 0.076	0.805 ± 0.064	0.826 ± 0.039	0.868 ± 0.039
Liver	8.383 ± 0.764	8.525 ± 1.012	8.563 ± 0.646	9.205 ± 1.385	9.982 ± 0.282	10.01 ± 0.872
Spleen	0.432 ± 0.101	0.490 ± 0.123	0.515 ± 0.051	0.506 ± 0.067	0.500 ± 0.018	0.501 ± 0.082
Lung	1.225 ± 1.104	1.269 ± 0.100	1.375 ± 0.129	1.374 ± 0.088	1.350 ± 0.084	1.374 ± 0.100
Kidney	0.900 ± 0.161	1.016 ± 0.267	1.028 ± 0.032	1.015 ± 0.181	1.012 ± 0.082	1.009 ± 0.144
Thymus	0.298 ± 0.021	0.335 ± 0.051	0.341 ± 0.072	0.339 ± 0.059	0.352 ± 0.032	0.338 ± 0.052
Adrenal gland	0.027 ± 0.003	0.025 ± 0.005	0.032 ± 0.002	0.029 ± 0.006	0.031 ± 0.002	0.030 ± 0.006
Uterus	0.512 ± 0.025	0.524 ± 0.017	0.511 ± 0.021	0.514 ± 0.020	0.536 ± 0.031	0.546 ± 0.045
Ovary	0.047 ± 0.004	0.049 ± 0.005	0.049 ± 0.004	0.051 ± 0.003	0.054 ± 0.005	0.053 ± 0.004

Measurements are given as the mean ± SD. Group 1: Control for acute group; Group 2: Acute group; Group 3: Control for subchronic group; Group 4: Subchronic group; Group 5: Control for chronic group; Group 6: Chronic group.

Table 3 Selected hematology analyses of rats in toxicity study

	Group 1	Group 2	Group 3	Group 4	Group 5	Group 6
RBC (× 1012/L)	7.196 ± 0.090	7.225 ± 0.340	7.346 ± 0.418	7.462 ± 0.430	7.276 ± 0.421	7.300 ± 0.347
HCT (%)	39.32 ± 0.740	38.98 ± 1.992	39.08 ± 0.958	38.50 ± 2.253	38.90 ± 2.220	37.05 ± 0.576
MCV (fL)	54.64 ± 0.541	52.28 ± 0.784	53.28 ± 1.767	53.34 ± 0.845	53.00 ± 0.714	53.04 ± 1.681
MCH (pg)	18.66 ± 0.114	18.01 ± 0.470	18.10 ± 0.765	18.39 ± 0.341	17.82 ± 1.043	18.51 ± 0.905
MCHC (g/dL)	34.18 ± 0.523	34.42 ± 0.480	33.96 ± 0.493	34.50 ± 0.245	34.48 ± 0.590	35.12 ± 0.545
RDW (%)	17.42 ± 0.444	17.87 ± 0.912	17.78 ± 0.691	17.29 ± 1.152	17.30 ± 0.570	17.92 ± 0.651
RETIC (K/μL)	2.86 ± 0.434	3.78 ± 0.630	2.78 ± 0.691	2.93 ± 0.706	2.48 ± 0.798	2.81 ± 0.802
RETIC	214.7 ± 35.25	270.6 ± 39.98	202.2 ± 42.64	216.6 ± 49.50	162.3 ± 5.118	178.0 ± 19.04
WBC (×1012/L)	7.644 ± 1.668	6.932 ± 2.50	7.592 ± 2.96	7.504 ± 2.121	6.440 ± 1.248	5.883 ± 2.897
NEU (%)	13.72 ± 4.217	17.50 ± 5.977	12.48 ± 2.104	15.40 ± 5.918	12.10 ± 2.351	14.66 ± 2.831
LYM (%)	75.06 ± 5.697	73.91 ± 6.311	80.36 ± 4.360	79.33 ± 7.194	78.88 ± 3.440	76.26 ± 2.858
MONO (%)	5.180 ± 0.832	6.800 ± 0.902	4.520 ± 1.117	5.540 ± 1.169	4.540 ± 0.963	6.160 ± 1.190
EOS (%)	0.60 ± 0.123	0.80 ± 0.340	0.80 ± 0.200	0.53 ± 0.200	0.62 ± 0.356	0.61 ± 0.166
BASO (%)	0.14 ± 0.114	0.25 ± 0.151	0.20 ± 0.141	0.18 ± 0.140	0.16 ± 0.114	0.19 ± 0.171
NEU (× 109/L)	1.024 ± 0.288	1.116 ± 0.495	0.976 ± 0.153	1.011 ± 0.468	1.096 ± 0.163	1.000 ± 0.096
LYM (× 109/L)	6.178 ± 1.593	4.849 ± 2.150	6.158 ± 2.684	6.002 ± 2.089	5.672 ± 0.854	5.398 ± 1.097
MONO (× 109/L)	0.386 ± 0.042	0.455 ± 0.161	0.426 ± 0.083	0.415 ± 0.120	0.406 ± 0.067	0.346 ± 0.111
EOS (× 109/L)	0.046 ± 0.011	0.05 ± 0.017	0.050 ± 0.029	0.037 ± 0.016	0.004 ± 0.012	0.034 ± 0.013
BASO (× 109/L)	0.01 ± 0.007	0.014 ± 0.010	0.001 ± 0.007	0.013 ± 0.07	0.016 ± 0.009	0.016 ± 0.012
PLT (K/μL)	1001 ± 193.2	1107 ± 178.6	1032 ± 142.8	974.4 ± 121.9	1169 ± 74.37	1075 ± 45.99
MPV (fL)	8.50 ± 0.100	8.43 ± 0.216	8.42 ± 0.148	8.63 ± 0.095	8.44 ± 0.089	8.47 ± 0.206
PDW (fL)	8.50 ± 0.158	8.62 ± 0.148	8.46 ± 0.422	8.30 ± 0.133	8.42 ± 0.303	8.71 ± 0.778
PCT (%)	1.037 ± 0.038	0.962 ± 0.137	0.870 ± 0.129	0.80 ± 0.151	0.844 ± 0.167	0.88 ± 0.107

Note: Measurements are given as the mean ± SD. RBC: Red blood cells; HCT: Hematocritt; MCV: Average red blood cell volume; MCH: Average hemoglobin concentration; RDW: Red blood cell distribution width; RETIC: Reticulocytes; WBC: White blood cells; NEU: Neutrophils; LYM: Lymphocytes; MONO: Monocytes; EOS: Eosinophils; BASO: Basophils; PLT: Platelet; MPV: Mean platelet volume; PDW: Platelet distribution width.

Table 4 Selected biochemistry analyses of rats in toxicity study

	Group 1	Group 2	Group3	Group 4	Group 5	Group 6
Urea	6.220 ± 0.512	6.260 ± 0.657	5.860 ± 0.493	6.19 ± 0.659	5.840 ± 0.270	6.380 ± 0.570
CREA	27.60 ± 5.128	32.30 ± 6.430	28.40 ± 4.722	26.40 ± 2.011	31.00 ± 6.557	31.10 ± 6.367
BUN/CREA	55.80 ± 11.71	48.90 ± 7.279	55.20 ± 8.899	58 ± 8.679	56.60 ± 5.771	57.80 ± 3.967
TP	60.8 ± 4.087	62.20 ± 5.673	61.80 ± 7.014	57.40 ± 2.914	60.20 ± 3.962	60.30 ± 5.165
ALB	31.80 ± 2.683	32.4 ± 4.060	36.80 ± 3.899	30.2 ± 3.584	30.00 ± 2.739	32.90 ± 3.695
GLOB	29.40 ± 1.817	29.8 ± 2.300	25.90 ± 3.194	27.4 ± 1.350	26.00 ± 3.674	27.80 ± 2.201
ALT	35.00 ± 6.042	34.7 ± 5.539	31.40 ± 8.355	38.30 ± 3.889	34.60 ± 7.162	36.30 ± 6.897
AST	73.40 ± 11.84	66.6 ± 14.010	70.20 ± 7.662	75.2 ± 15.050	64.20 ± 16.68	61.4 ± 13.16
ALKP	108 ± 25.03	121.7 ± 32.840	107.0 ± 37.36	100.3 ± 11.910	108.4 ± 23.39	95.20 ± 11.70
Na	142.6 ± 1.949	141.2 ± 2.348	143.2 ± 1.483	141.7 ± 2.627	142.2 ± 1.643	144.1 ± 2.234
K	5.180 ± 0.148	5.230 ± 0.457	4.820 ± 0.601	5.01 ± 0.213	4.700 ± 0.245	5.96 ± 0.390
CL	103.8 ± 2.387	106.2 ± 3.293	104.2 ± 2.387	106.6 ± 3.084	103.8 ± 2.775	105.4 ± 5.719

Note: Measurements are given as the mean ± SD. CREA: Creatinine; TP: Total protein; ALB: Albumin; GLOB: Globulin; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; ALKP: Alkaline phosphatase; BUN: Blood urea nitrogen.